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Literature summary for 3.2.1.80 extracted from

  • Lothier, J.; Lasseur, B.; Le Roy, K.; Van Laere, A.; Prudhomme, M.P.; Barre, P.; Van den Ende, W.; Morvan-Bertrand, A.
    Cloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilization (2007), J. Exp. Bot., 58, 1969-1983.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X33 Lolium perenne

Inhibitors

Inhibitors Comment Organism Structure
additional information glucose and fructose (20 mM) do not affect 1-FEHa activity Lolium perenne
sucrose 90% inhibition at 20 mM Lolium perenne

Organism

Organism UniProt Comment Textmining
Lolium perenne Q64GB3 variant Bravo
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G50 gel filtration Lolium perenne

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Lolium perenne
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1-kestotetraose + H2O 100% activity Lolium perenne ?
-
 ?
1-kestotriose + H2O 58% activity compared to 1,1-kestotetraose Lolium perenne ?
-
 ?
6-kestotriose + H2O 6% activity compared to 1,1-kestotetraose Lolium perenne ?
-
 ?
6G-kestotriose + H2O 19% activity compared to 1,1-kestotetraose Lolium perenne ?
-
 ?
inulin + 5 H2O 24% activity compared to 1,1-kestotetraose Lolium perenne 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
levan + H2O 0.5% activity compared to 1,1-kestotetraose Lolium perenne D-fructose + ?
-
 ?
additional information no hydrolase activity towards sucrose and loliose Lolium perenne ?
-
 ?

Synonyms

Synonyms Comment Organism
1-FEHa
-
 Lolium perenne
EC 3.2.1.153 formerly Lolium perenne
fructan 1-exohydrolase
-
 Lolium perenne

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 50 20% of the maximal activity is still detected at 4°C, 1-FEHa activity decreases strongly above 40°C and falls to 20% of ist maximal activity at 50°C Lolium perenne

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Lolium perenne

pI Value

Organism Comment pI Value Maximum pI Value
Lolium perenne isoelectric focusing
-
 5.2