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Literature summary for 3.2.1.80 extracted from

  • Uzunova, K.; Vassileva, A.; Kambourova, M.; Ivanova, V.; Spasova, D.; Mandeva, R.; Derekova, A.; Tonkova, A.
    Production and properties of a bacterial thermostable exo-inulinase (2001), Z. Naturforsch. C, 56, 1022-1028.
    View publication on PubMed
Search for more literature for 3.2.1.80

Application

Application Comment Organism
nutrition microbial production of ultra high fructose glucose syrups Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) 11
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
 Bacillus sp. (in: Bacteria)

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration, 1.3fold Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 19.0 ?mol/min/ml, for sucrose, pH 7, at 60ºC Bacillus sp. (in: Bacteria)
additional information
-
 2.0 ?mol/min/ml, for inulin, pH 7, at 60ºC Bacillus sp. (in: Bacteria)
additional information
-
 6.2 ?mol/min/ml, for raffinose, pH 7, at 60ºC Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + 5 H2O no activity with melizitose Bacillus sp. (in: Bacteria) 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
inulin + 5 H2O no activity with melizitose Bacillus sp. (in: Bacteria) 11 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
raffinose + H2O
-
 Bacillus sp. (in: Bacteria) beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?
raffinose + H2O
-
 Bacillus sp. (in: Bacteria) 11 beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?
sucrose + H2O
-
 Bacillus sp. (in: Bacteria) beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
sucrose + H2O
-
 Bacillus sp. (in: Bacteria) 11 beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Synonyms

Synonyms Comment Organism
exoinulinase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 without inulin, 73% of maximal activity after heating for 60 min Bacillus sp. (in: Bacteria)
60
-
 without inulin, 78% of maximal activity after heating for 15 min Bacillus sp. (in: Bacteria)
65
-
 with inulin, 92% of maximal activity after heating for 60 min Bacillus sp. (in: Bacteria)
65
-
 with inulin, 97% of maximal activity after heating for 15 min Bacillus sp. (in: Bacteria)
65
-
 without inulin, 17% of maximal activity after heating for 60 min Bacillus sp. (in: Bacteria)
65
-
 without inulin, 34% of maximal activity after heating for 15 min Bacillus sp. (in: Bacteria)
70
-
 with inulin, 56% of maximal activity after heating for 60 min Bacillus sp. (in: Bacteria)
70
-
 with inulin, 85% of maximal activity after heating for 15 min Bacillus sp. (in: Bacteria)
70
-
 without inulin, 2% of maximal activity after heating for 60 min Bacillus sp. (in: Bacteria)
70
-
 without inulin, 7% of maximal activity after heating for 15 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 at pH 8.0, 58% of maximal activity Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7.5 95-100% of maximal activity retained Bacillus sp. (in: Bacteria)