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Literature summary for 3.2.1.80 extracted from
- Purification and preliminary characterization of exo-beta-D-fructosidase in Streptococcus salivarius KTA-19 (1985), Infect. Immun., 47, 271-276.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | slight stimulatuion after 30 min of incubation | Streptococcus salivarius |  |
| KCN | slight stimulatuion after 30 min of incubation | Streptococcus salivarius | |
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing accelerates inactivation | Streptococcus salivarius |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Streptococcus salivarius | |
| Cu2+ | - |
Streptococcus salivarius | |
| Fe3+ | - |
Streptococcus salivarius | |
| Hg2+ | - |
Streptococcus salivarius | |
| p-chloromercuribenzoate | presence of cysteine protects against inactivation but no recovery | Streptococcus salivarius | |
| Tris | - |
Streptococcus salivarius | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | slight stimulation | Streptococcus salivarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | 85000 | gel filtration, SDS-PAGE | Streptococcus salivarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus salivarius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus salivarius |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.02 mg/ml, 20 mM phosphate buffer, pH 7.0, stable at least for several days, 90% loss of activity after 2 weeks | Streptococcus salivarius |
| higher stability at -20°C than at 4°C | Streptococcus salivarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | - |
Streptococcus salivarius | D-fructose + ? | - |
? | |
| levan + H2O | - |
Streptococcus salivarius | D-fructose + ? | - |
? | |
| additional information | not: melizitose, dextran, pseudonigeran | Streptococcus salivarius | ? | - |
? | |
| raffinose + H2O | - |
Streptococcus salivarius | D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside | - |
? | |
| sucrose + H2O | - |
Streptococcus salivarius | D-fructose + D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Streptococcus salivarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 55 | rapid inactivation at 60°C | Streptococcus salivarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 7.0, 30 min | Streptococcus salivarius |
| 60 | - |
rapid inactivation | Streptococcus salivarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptococcus salivarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | - |
Streptococcus salivarius |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
almost complete loss of activity at | Streptococcus salivarius |
| 7 | - |
37°C, 30 min | Streptococcus salivarius |
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