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Literature summary for 3.2.1.80 extracted from

  • Burne, R.A.; Schilling, K.; Bowen, W.H.; Yasbin, R.E.
    Expression, purification, and characterization of an exo-beta-D-fructosidase of Streptococcus mutans (1987), J. Bacteriol., 169, 4507-4517.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.80

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus mutans

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Streptococcus mutans
Cu2+ partially Streptococcus mutans
Hg2+
-
 Streptococcus mutans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slight stimulation Streptococcus mutans
Mn2+ slight stimulation Streptococcus mutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 x * 140000, SDS-PAGE Streptococcus mutans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fructans + H2O Streptococcus mutans degradation D-fructose
-
 ?
fructans + H2O Streptococcus mutans GS-5 degradation D-fructose
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
 GS-5
-
Streptococcus mutans GS-5
-
 GS-5
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fructans + H2O degradation Streptococcus mutans D-fructose
-
 ?
fructans + H2O degradation Streptococcus mutans GS-5 D-fructose
-
 ?
inulin + H2O
-
 Streptococcus mutans D-fructose + ?
-
 ?
inulin + H2O
-
 Streptococcus mutans GS-5 D-fructose + ?
-
 ?
levan + H2O
-
 Streptococcus mutans D-fructose + ?
-
 ?
levan + H2O
-
 Streptococcus mutans GS-5 D-fructose + ?
-
 ?
raffinose + H2O
-
 Streptococcus mutans D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
 ?
raffinose + H2O
-
 Streptococcus mutans GS-5 D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
-
 ?
sucrose + H2O
-
 Streptococcus mutans D-fructose + D-glucose
-
 ?
sucrose + H2O
-
 Streptococcus mutans GS-5 D-fructose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 140000, SDS-PAGE Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 sucrose Streptococcus mutans
5.5
-
 fructan polymers Streptococcus mutans

pH Range

pH Minimum pH Maximum Comment Organism
3.5 7 little fructanase activity at or above pH 7.0 Streptococcus mutans