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Literature summary for 3.2.1.8 extracted from
- Delineating thermophilic xylanase from Bacillus licheniformis DM5 towards its potential application in xylooligosaccharides production (2019), World J. Microbiol. Biotechnol., 35, 34 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | Bacillus licheniformis strain DM5 is attributed for the production of prebiotic and anti-inflammatory XOS from agrowaste | Bacillus licheniformis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km is 1.5 mg/ml with substrate beechwood xylan | Bacillus licheniformis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus licheniformis | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
isolated from Garampani hotspring, Assam, India | - |
| Bacillus licheniformis DM5 | - |
isolated from Garampani hotspring, Assam, India | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular xylanase partially by ammonium sulfate fractionation and dialysis | Bacillus licheniformis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | highest production of xylanase from strain DM5 is achieved in the TY medium with cell densities 12 g/l and extracellular protein concentration containing xylanase 400 mg/l | Bacillus licheniformis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the purified extrecllular enzyme shows maximum activity 2.7 U/ml against beechwood xylan, 2.3 U/ml with birchwood xylan, 1.2 U/ml with glucuronoxylan, and 0.8 U/ml with arabinoxylan | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arabinoxylan + H2O | a branched chain xylan | Bacillus licheniformis | ? | - |
? |  |
| arabinoxylan + H2O | a branched chain xylan | Bacillus licheniformis DM5 | ? | - |
? | |
| beechwood xylan + H2O | best substrate | Bacillus licheniformis | xylose + xylotriose + xylobiose | - |
? | |
| beechwood xylan + H2O | best substrate | Bacillus licheniformis DM5 | xylose + xylotriose + xylobiose | - |
? | |
| birchwood xylan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| birchwood xylan + H2O | - |
Bacillus licheniformis DM5 | ? | - |
? | |
| glucuronoxylan + H2O | a branched chain xylan | Bacillus licheniformis | ? | - |
? | |
| glucuronoxylan + H2O | a branched chain xylan | Bacillus licheniformis DM5 | ? | - |
? | |
| additional information | the enzyme shows exo-acting activity against synthetic 4-nitrophenyl xylopyranoside substrates. Molecular interaction analysis of the enzyme with xylobiose, xylotriose, xylotetraose, xylopentaose, and xylohexose, and of xyloologisaccharides with the sugar binding domain (BIAXBP) of probiotic strains, detailed overview. The purified extrecllular enzyme shows maximum activity 2.7 U/ml against beechwood xylan, 2.3 U/ml with birchwood xylan, 1.2 U/ml with glucuronoxylan, and 0.8 U/ml with arabinoxylan | Bacillus licheniformis | ? | - |
? | |
| additional information | the enzyme shows exo-acting activity against synthetic 4-nitrophenyl xylopyranoside substrates. Molecular interaction analysis of the enzyme with xylobiose, xylotriose, xylotetraose, xylopentaose, and xylohexose, and of xyloologisaccharides with the sugar binding domain (BIAXBP) of probiotic strains, detailed overview. The purified extrecllular enzyme shows maximum activity 2.7 U/ml against beechwood xylan, 2.3 U/ml with birchwood xylan, 1.2 U/ml with glucuronoxylan, and 0.8 U/ml with arabinoxylan | Bacillus licheniformis DM5 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, native extracellular enzyme, SDS-PAGE | Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus licheniformis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 75 | activity range, profile overview | Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
partially purified extracellular xylanase, completely stable for 30 min, loss of 50% activity after 120 min | Bacillus licheniformis |
| 60 | - |
partially purified extracellular xylanase, loss of 10% activity after 60 min, loss of 55% activity after 120 min | Bacillus licheniformis |
| 70 | - |
partially purified extracellular xylanase, loss of 80% activity after 120 min | Bacillus licheniformis |
| 80 | - |
partially purified extracellular xylanase, loss of 85% activity after 120 min | Bacillus licheniformis |
| 90 | - |
partially purified extracellular xylanase, loss of 90% activity after 120 min | Bacillus licheniformis |
| 100 | - |
partially purified extracellular xylanase, inactivation after 120 min | Bacillus licheniformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.89 | - |
arabinoxylan | pH 7.5, 50°C | Bacillus licheniformis | |
| 1.03 | - |
glucuronoxylan | pH 7.5, 50°C | Bacillus licheniformis | |
| 1.7 | - |
birchwood xylan | pH 7.5, 50°C | Bacillus licheniformis | |
| 1.8 | - |
beechwood xylan | pH 7.5, 50°C | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | activity range, profile overview | Bacillus licheniformis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzymatic hydrolysis of beechwood xylan and preprocessed agrowaste corncob leads to high release of xylotriose and xylobiose oligosaccharide (XOS). Xylobiose and xylotriose exhibit higher binding affinities with the BIAXP transporter protein of probiotic bacteria explaining their easy uptake by the cells. MIxed xylooligosaccharides are cytotoxic to HeLa cells. Bacillus licheniformis strain DM5 is attributed for the production of prebiotic and anti-inflammatory XOS from agrowaste | Bacillus licheniformis |
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