Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme displays an excellent tolerance to trypsin and pepsin proteases but is degraded by proteinase K, and slightly by alkaline protease | Aspergillus flavus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Aspergillus flavus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beechwood xylan + H2O | Aspergillus flavus | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | xylobiose + xylopentaose + ? | - |
? | |
beechwood xylan + H2O | Aspergillus flavus SRRC 167 | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | xylobiose + xylopentaose + ? | - |
? | |
beechwood xylan + H2O | Aspergillus flavus ATCC 200026 | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | xylobiose + xylopentaose + ? | - |
? | |
beechwood xylan + H2O | Aspergillus flavus FGSC A1120 | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | xylobiose + xylopentaose + ? | - |
? | |
beechwood xylan + H2O | Aspergillus flavus NRRL 3357 | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | xylobiose + xylopentaose + ? | - |
? | |
beechwood xylan + H2O | Aspergillus flavus JCM 12722 | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | xylobiose + xylopentaose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus flavus | B8NJ86 | - |
- |
Aspergillus flavus ATCC 200026 | B8NJ86 | - |
- |
Aspergillus flavus FGSC A1120 | B8NJ86 | - |
- |
Aspergillus flavus JCM 12722 | B8NJ86 | - |
- |
Aspergillus flavus NRRL 3357 | B8NJ86 | - |
- |
Aspergillus flavus SRRC 167 | B8NJ86 | - |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme from crude culture supernatant 1.7fold by ammonium sulfate fractionation, dialysis, and gel filtration, to homogeneity | Aspergillus flavus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | production of the xylanase by Aspergillus flavus using corncobs as the sole carbon source in shake-flask cultures is monitored at 35°C for 5 day | Aspergillus flavus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
383.4 | - |
purified native extracellular enzyme, pH 7.5, 55°C, substrate beechwood xylan | Aspergillus flavus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beechwood xylan + H2O | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | Aspergillus flavus | xylobiose + xylopentaose + ? | - |
? | |
beechwood xylan + H2O | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | Aspergillus flavus SRRC 167 | xylobiose + xylopentaose + ? | - |
? | |
beechwood xylan + H2O | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | Aspergillus flavus ATCC 200026 | xylobiose + xylopentaose + ? | - |
? | |
beechwood xylan + H2O | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | Aspergillus flavus FGSC A1120 | xylobiose + xylopentaose + ? | - |
? | |
beechwood xylan + H2O | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | Aspergillus flavus NRRL 3357 | xylobiose + xylopentaose + ? | - |
? | |
beechwood xylan + H2O | the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose | Aspergillus flavus JCM 12722 | xylobiose + xylopentaose + ? | - |
? | |
additional information | AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose | Aspergillus flavus | ? | - |
? | |
additional information | AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose | Aspergillus flavus SRRC 167 | ? | - |
? | |
additional information | AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose | Aspergillus flavus ATCC 200026 | ? | - |
? | |
additional information | AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose | Aspergillus flavus FGSC A1120 | ? | - |
? | |
additional information | AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose | Aspergillus flavus NRRL 3357 | ? | - |
? | |
additional information | AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose | Aspergillus flavus JCM 12722 | ? | - |
? | |
xylotetraose + H2O | - |
Aspergillus flavus | 2 xylobiose | - |
? | |
xylotetraose + H2O | - |
Aspergillus flavus SRRC 167 | 2 xylobiose | - |
? | |
xylotetraose + H2O | - |
Aspergillus flavus ATCC 200026 | 2 xylobiose | - |
? | |
xylotetraose + H2O | - |
Aspergillus flavus FGSC A1120 | 2 xylobiose | - |
? | |
xylotetraose + H2O | - |
Aspergillus flavus NRRL 3357 | 2 xylobiose | - |
? | |
xylotetraose + H2O | - |
Aspergillus flavus JCM 12722 | 2 xylobiose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32200, SDS-PAGE | Aspergillus flavus |
Synonyms | Comment | Organism |
---|---|---|
AfXynB | - |
Aspergillus flavus |
beta-1, 4-endoxylanase | - |
Aspergillus flavus |
xlnB | - |
Aspergillus flavus |
XynB | - |
Aspergillus flavus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Aspergillus flavus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 63 | over 50% of maximal activity within this range, profile overview | Aspergillus flavus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | purified native, 30% activity remaining | Aspergillus flavus |
30 | 50 | purified native, stable at | Aspergillus flavus |
60 | - |
purified native, inactivation | Aspergillus flavus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Aspergillus flavus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | over 50% of maximal activity within this range, profile overview | Aspergillus flavus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9.5 | purified native, over 80% activity remaining | Aspergillus flavus |