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Literature summary for 3.2.1.8 extracted from

  • Chen, Z.; Liu, Y.; Zaky, A.A.; Liu, L.; Chen, Y.; Li, S.; Jia, Y.
    Characterization of a novel xylanase from Aspergillus flavus with the unique properties in production of xylooligosaccharides (2019), J. Basic Microbiol., 59, 351-358 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme displays an excellent tolerance to trypsin and pepsin proteases but is degraded by proteinase K, and slightly by alkaline protease Aspergillus flavus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Aspergillus flavus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beechwood xylan + H2O Aspergillus flavus the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose xylobiose + xylopentaose + ?
-
?
beechwood xylan + H2O Aspergillus flavus SRRC 167 the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose xylobiose + xylopentaose + ?
-
?
beechwood xylan + H2O Aspergillus flavus ATCC 200026 the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose xylobiose + xylopentaose + ?
-
?
beechwood xylan + H2O Aspergillus flavus FGSC A1120 the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose xylobiose + xylopentaose + ?
-
?
beechwood xylan + H2O Aspergillus flavus NRRL 3357 the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose xylobiose + xylopentaose + ?
-
?
beechwood xylan + H2O Aspergillus flavus JCM 12722 the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose xylobiose + xylopentaose + ?
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?

Organism

Organism UniProt Comment Textmining
Aspergillus flavus B8NJ86
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Aspergillus flavus ATCC 200026 B8NJ86
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Aspergillus flavus FGSC A1120 B8NJ86
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-
Aspergillus flavus JCM 12722 B8NJ86
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-
Aspergillus flavus NRRL 3357 B8NJ86
-
-
Aspergillus flavus SRRC 167 B8NJ86
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-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme from crude culture supernatant 1.7fold by ammonium sulfate fractionation, dialysis, and gel filtration, to homogeneity Aspergillus flavus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium production of the xylanase by Aspergillus flavus using corncobs as the sole carbon source in shake-flask cultures is monitored at 35°C for 5 day Aspergillus flavus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
383.4
-
purified native extracellular enzyme, pH 7.5, 55°C, substrate beechwood xylan Aspergillus flavus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose Aspergillus flavus xylobiose + xylopentaose + ?
-
?
beechwood xylan + H2O the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose Aspergillus flavus SRRC 167 xylobiose + xylopentaose + ?
-
?
beechwood xylan + H2O the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose Aspergillus flavus ATCC 200026 xylobiose + xylopentaose + ?
-
?
beechwood xylan + H2O the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose Aspergillus flavus FGSC A1120 xylobiose + xylopentaose + ?
-
?
beechwood xylan + H2O the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose Aspergillus flavus NRRL 3357 xylobiose + xylopentaose + ?
-
?
beechwood xylan + H2O the endoxylanase hydrolyzes beechwood xylan to only yield xylobiose (X2) and xylopentaose Aspergillus flavus JCM 12722 xylobiose + xylopentaose + ?
-
?
additional information AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose Aspergillus flavus ?
-
?
additional information AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose Aspergillus flavus SRRC 167 ?
-
?
additional information AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose Aspergillus flavus ATCC 200026 ?
-
?
additional information AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose Aspergillus flavus FGSC A1120 ?
-
?
additional information AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose Aspergillus flavus NRRL 3357 ?
-
?
additional information AfXynB rapidly hydrolyzes xylotetraose and slightly hydrolyze xylotriose to xylobiose as the major end product without formation of the corresponding amount of xylose, but hardly hydrolyzes xylobiose. No activity with 4-nitrophenyl beta-D-xylopyranoside and carboxymethyl cellulose Aspergillus flavus JCM 12722 ?
-
?
xylotetraose + H2O
-
Aspergillus flavus 2 xylobiose
-
?
xylotetraose + H2O
-
Aspergillus flavus SRRC 167 2 xylobiose
-
?
xylotetraose + H2O
-
Aspergillus flavus ATCC 200026 2 xylobiose
-
?
xylotetraose + H2O
-
Aspergillus flavus FGSC A1120 2 xylobiose
-
?
xylotetraose + H2O
-
Aspergillus flavus NRRL 3357 2 xylobiose
-
?
xylotetraose + H2O
-
Aspergillus flavus JCM 12722 2 xylobiose
-
?

Subunits

Subunits Comment Organism
? x * 32200, SDS-PAGE Aspergillus flavus

Synonyms

Synonyms Comment Organism
AfXynB
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Aspergillus flavus
beta-1, 4-endoxylanase
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Aspergillus flavus
xlnB
-
Aspergillus flavus
XynB
-
Aspergillus flavus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Aspergillus flavus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 63 over 50% of maximal activity within this range, profile overview Aspergillus flavus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 purified native, 30% activity remaining Aspergillus flavus
30 50 purified native, stable at Aspergillus flavus
60
-
purified native, inactivation Aspergillus flavus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
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-
Aspergillus flavus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 over 50% of maximal activity within this range, profile overview Aspergillus flavus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9.5 purified native, over 80% activity remaining Aspergillus flavus