Literature summary for 3.2.1.8 extracted from

Adiguzel, G.; Faiz, O.; Sisecioglu, M.; Sari, B.; Baltaci, O.; Akbulut, S.; Genc, B.; Adiguzel, A.
A novel endo-beta-1,4-xylanase from Pediococcus acidilactici GC25, purification, characterization and application in clarification of fruit juices (2019), Int. J. Biol. Macromol., 129, 571-578 .

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Application

Application	Comment	Organism
food industry	fruit juice clarification potential of GC25 xylanase at mild conditions. Pediococcus acidilactici strain GC25 xylanase causes a high increase in reducing sugar content after 30 min incubation at 40°C	Pediococcus acidilactici

Cloned(Commentary)

Cloned (Comment)	Organism
gene xynA	Pediococcus acidilactici

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Km value of the purified GC25 xylanase is 3.10 mg/ml	Pediococcus acidilactici

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Pediococcus acidilactici	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	no or poor effects by K+, Cd2+, Ba2+, Co2+, Sr2+, Mg2+, Ca2+, Al3+, Zn2+, and Ni2+	Pediococcus acidilactici

Organism

Organism	UniProt	Comment	Textmining
Pediococcus acidilactici	-	-	-
Pediococcus acidilactici GC25	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native extracellular enzyme 4.6fold by acetone precipitation, anion exchange and cation exchange chromatography	Pediococcus acidilactici

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
318.45	-	purified native enzyme, pH 7.0, 40°C	Pediococcus acidilactici

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	clarification of fruit juice with Pediococcus acidilactici GC25 xylanase, overview	Pediococcus acidilactici	?	-	?
additional information	clarification of fruit juice with Pediococcus acidilactici GC25 xylanase, overview	Pediococcus acidilactici GC25	?	-	?

Synonyms

Synonyms	Comment	Organism
endo-beta-1,4-xylanase	-	Pediococcus acidilactici
GC25 xylanase	-	Pediococcus acidilactici
XynA	-	Pediococcus acidilactici

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Pediococcus acidilactici

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	80	native extracellular enzyme, over 65% activity within this range, profile overview	Pediococcus acidilactici

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	50	purified extracellular enzyme, 2 h, over 80% activity remaining	Pediococcus acidilactici
60	-	purified extracellular enzyme, 2 h, loss of 80% activity, half-life is 20 min	Pediococcus acidilactici

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Pediococcus acidilactici

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	9	native extracellular enzyme, over 50% activity within this range, profile overview	Pediococcus acidilactici

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	-	purified extracellular enzyme, 85% activity remaining after 24 h, 50% activity remaining after 168 h	Pediococcus acidilactici
5	-	purified extracellular enzyme, 85% activity remaining after 24 h, 70% activity remaining after 168 h	Pediococcus acidilactici
7	-	purified extracellular enzyme, 95% activity remaining after 24 h, 80% activity remaining after 168 h	Pediococcus acidilactici
9	-	purified extracellular enzyme, 90% activity remaining after 24 h, 80% activity remaining after 168 h	Pediococcus acidilactici

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Release 2023.1 (January 2023)