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Literature summary for 3.2.1.8 extracted from

  • Basit, A.; Liu, J.; Miao, T.; Zheng, F.; Rahim, K.; Lou, H.; Jiang, W.
    Characterization of two endo-beta-1, 4-xylanases from Myceliophthora thermophila and their saccharification efficiencies, synergistic with commercial cellulase (2018), Front. Microbiol., 9, 233 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme activity is not affected by EDTA Thermothelomyces thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
gene MYCTH_49824, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X33 under control of an AOX1 promoter Thermothelomyces thermophilus
gene MYCTH_56237, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X33 under control of an AOX1 promoter Thermothelomyces thermophilus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Thermothelomyces thermophilus
additional information the enzyme activity is not affected by EDTA; the enzyme activity is not affected by EDTA Thermothelomyces thermophilus
Ni2+
-
 Thermothelomyces thermophilus
Zn2+
-
 Thermothelomyces thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km is 5.67 mg/ml Thermothelomyces thermophilus
additional information
-
 additional information Km is 8.80 mg/ml Thermothelomyces thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activates Thermothelomyces thermophilus
Mn2+ activates Thermothelomyces thermophilus

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus G2QDB9 i.e. Sporotrichum thermophile
-
Thermothelomyces thermophilus G2QIK8 i.e. Sporotrichum thermophile
-
Thermothelomyces thermophilus ATCC 42464 G2QDB9 i.e. Sporotrichum thermophile
-
Thermothelomyces thermophilus ATCC 42464 G2QIK8 i.e. Sporotrichum thermophile
-
Thermothelomyces thermophilus BCRC 31852 G2QDB9 i.e. Sporotrichum thermophile
-
Thermothelomyces thermophilus BCRC 31852 G2QIK8 i.e. Sporotrichum thermophile
-
Thermothelomyces thermophilus DSM 1799 G2QDB9 i.e. Sporotrichum thermophile
-
Thermothelomyces thermophilus DSM 1799 G2QIK8 i.e. Sporotrichum thermophile
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein MYCTH_49824 has one O-glycosylation site (at residue 40) and four N-glycosylation sites (resides 20, 33, 101, 107) Thermothelomyces thermophilus
glycoprotein MYCTH_56237 has two putative O-glycosylation sites (residues 35 and 37) and one N-glycosylation site (Asn22) Thermothelomyces thermophilus

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Pichia pastoris strain X33 by nickel affinity chromatography Thermothelomyces thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1412.5
-
 purified recombinant enzyme, pH 7.0, 60°C Thermothelomyces thermophilus
1533.7
-
 purified recombinant enzyme, pH 6.0, 60°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme significantly cooperates with a commercial cellulase, it perfoms saccharification of the pretreated corn stover Thermothelomyces thermophilus ?
-
 ?
additional information the enzyme significantly cooperates with a commercial cellulase, it perfoms saccharification of the pretreated corn stover Thermothelomyces thermophilus ATCC 42464 ?
-
 ?
additional information the enzyme significantly cooperates with a commercial cellulase, it perfoms saccharification of the pretreated corn stover Thermothelomyces thermophilus BCRC 31852 ?
-
 ?
additional information the enzyme significantly cooperates with a commercial cellulase, it perfoms saccharification of the pretreated corn stover Thermothelomyces thermophilus DSM 1799 ?
-
 ?
xylan + H2O RBB-xylan as substrate Thermothelomyces thermophilus ?
-
 ?
xylan + H2O RBB-xylan as substrate Thermothelomyces thermophilus ATCC 42464 ?
-
 ?
xylan + H2O RBB-xylan as substrate Thermothelomyces thermophilus BCRC 31852 ?
-
 ?
xylan + H2O RBB-xylan as substrate Thermothelomyces thermophilus DSM 1799 ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-1, 4-xylanase
-
 Thermothelomyces thermophilus
MYCTH_49824
-
 Thermothelomyces thermophilus
MYCTH_56237
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 recombinant enzyme Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 purified recombinant enzyme, 30 min, 60% activity remaining Thermothelomyces thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 recombinant enzyme Thermothelomyces thermophilus
7
-
 recombinant enzyme Thermothelomyces thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 12 purified recombinant enzyme, 60 min, room temperature, MYCTH_49824 retains nearly 70% activity Thermothelomyces thermophilus
2 12 purified recombinant enzyme, 60 min, room temperature, MYCTH_56237 retains nearly 70% activity Thermothelomyces thermophilus

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 11, GH11 Thermothelomyces thermophilus
additional information multiple template-based enzyme structure homology modeling Thermothelomyces thermophilus