Any feedback?
Literature summary for 3.2.1.8 extracted from
- Improvement of thermostability and activity of Trichoderma reesei endo-xylanase Xyn III on insoluble substrates (2016), Appl. Microbiol. Biotechnol., 100, 8043-8051 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Trichoderma reesei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the addition of a xylan-binding domain to the C-terminus of Xyn III improves its hydrolytic activity on insoluble xylan | Trichoderma reesei |
| Q286A/N340Y | substantial improvement of thermostability | Trichoderma reesei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | LC132960 | codon-optimized gene | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.6 | - |
wild-type, substrate insoluble oat spelt xylan, pH 6.0, 50°C | Trichoderma reesei |
| 49.3 | - |
mutant Q286A/N340Y, substrate insoluble oat spelt xylan, pH 6.0, 50°C | Trichoderma reesei |
| 142 | - |
mutant Q286A/N340Y, substrate soluble oat spelt xylan, pH 6.0, 50°C | Trichoderma reesei |
| 143 | - |
wild-type, substrate soluble oat spelt xylan, pH 6.0, 50°C | Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| birchwood xylan + H2O | - |
Trichoderma reesei | ? | - |
? |  |
| insoluble oat spelt xylan + H2O | - |
Trichoderma reesei | ? | - |
? | |
| soluble oat spelt xylan + H2O | - |
Trichoderma reesei | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Xyn III | - |
Trichoderma reesei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
wild-type | Trichoderma reesei |
| 60 | - |
mutant Q286A/N340Y | Trichoderma reesei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
30 min, almost complete loss of activity for wild-type, 9% residual activity for mutant Q286A/N340Y | Trichoderma reesei |
| 60 | - |
30 min, 51% residual activity, mutant Q286A/N340Y | Trichoderma reesei |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
