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Literature summary for 3.2.1.8 extracted from
- Biochemical properties of xylanases from a thermophilic fungus, Melanocarpus albomyces, and their action on plant cell walls (1999), J. Biosci., 24, 461-470 .
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
larchwood xylan | Km value 0.33 mg/ml, isoform xylanase IA, pH 6.6, 50°C | Melanocarpus albomyces | |
| additional information | - |
larchwood xylan | Km value 1.69 mg/ml, isoform xylanase IIIA, pH 5.6, 50°C | Melanocarpus albomyces |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Melanocarpus albomyces | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Melanocarpus albomyces | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 7% carbohydrate content, isoform xylanase IA | Melanocarpus albomyces |
| no glycoprotein | isoform xylanase IIIA | Melanocarpus albomyces |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from bagasse-grown cultures | Melanocarpus albomyces |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 44 | - |
isoform xylanase IA, pH 6.6, 50°C | Melanocarpus albomyces |
| 200 | - |
isoform xylanase IIIA, pH 5.6, 50°C | Melanocarpus albomyces |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| delignified bagasse + H2O | - |
Melanocarpus albomyces | ? | - |
? |  |
| larchwood xylan + H2O | - |
Melanocarpus albomyces | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24000, SDS-PAGE, isoform xylanase IIIA | Melanocarpus albomyces |
| ? | x * 38000, SDS-PAGE, isoform xylanase IA | Melanocarpus albomyces |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
both isoforms xylanase IA and xylanase IIIA | Melanocarpus albomyces |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 5-10, stable for 24 h, both isoforms xylanase IA and xylanase IIIA | Melanocarpus albomyces |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
isoform xylanase IIIA | Melanocarpus albomyces |
| 6.6 | - |
isoform xylanase IA | Melanocarpus albomyces |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | 50°C, stable for 24 h, both isoforms xylanase IA and xylanase IIIA | Melanocarpus albomyces |
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