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Literature summary for 3.2.1.8 extracted from

  • Garcia-Huante, Y.; Cayetano-Cruz, M.; Santiago-Hernandez, A.; Cano-Ramirez, C.; Marsch-Moreno, R.; Campos, J.E.; Aguilar-Osorio, G.; Benitez-Cardoza, C.G.; Trejo-Estrada, S.; Hidalgo-Lara, M.E.
    The thermophilic biomass-degrading fungus Thielavia terrestris Co3Bag1 produces a hyperthermophilic and thermostable beta-1,4-xylanase with exo- and endo-activity (2017), Extremophiles, 21, 175-186 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 5 mM, 40% residual activity Thermothielavioides terrestris
EDTA 5 mM, 72% residual activity Thermothielavioides terrestris
Hg2+ 5 mM, no residual activity Thermothielavioides terrestris
Mg2+ 5 mM, 54% residual activity Thermothielavioides terrestris
Mn2+ 5 mM, 46% residual activity Thermothielavioides terrestris
Ni2+ 5 mM, 58% residual activity Thermothielavioides terrestris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
beechwood xylan Km value 0.41 mg/ml, pH 5.5, 85°C Thermothielavioides terrestris
additional information
-
birchwood xylan Km value 0.55 mg/ml, pH 5.5, 85°C Thermothielavioides terrestris
additional information
-
oat spelt xylan Km value 0.58 mg/ml, pH 5.5, 85°C Thermothielavioides terrestris
additional information
-
carboxymethyl cellulose Km value 6.6 mg/ml, pH 5.5, 85°C Thermothielavioides terrestris

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermothielavioides terrestris
-
-

Organism

Organism UniProt Comment Textmining
Thermothielavioides terrestris
-
-
-
Thermothielavioides terrestris Co3Bag1
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content about 20% Thermothielavioides terrestris

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
Thermothielavioides terrestris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
169.6
-
pH 5.5, 85°C Thermothielavioides terrestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O 2.2fold higher activity on carboxymethyl cellulose than on beechwood xylan Thermothielavioides terrestris ?
-
?
beechwood xylan + H2O 2.2fold higher activity on carboxymethyl cellulose than on beechwood xylan Thermothielavioides terrestris Co3Bag1 ?
-
?
birchwood xylan + H2O
-
Thermothielavioides terrestris ?
-
?
birchwood xylan + H2O
-
Thermothielavioides terrestris Co3Bag1 ?
-
?
carboxymethyl cellulose + H2O 2.2fold higher activity on carboxymethyl cellulose than on beechwood xylan Thermothielavioides terrestris ?
-
?
carboxymethyl cellulose + H2O 2.2fold higher activity on carboxymethyl cellulose than on beechwood xylan Thermothielavioides terrestris Co3Bag1 ?
-
?
additional information enzyme displays both endo- and exoglucanase activities. No substrate: avicel Thermothielavioides terrestris ?
-
?
additional information enzyme displays both endo- and exoglucanase activities. No substrate: avicel Thermothielavioides terrestris Co3Bag1 ?
-
?
oat spelt xylan + H2O
-
Thermothielavioides terrestris ?
-
?
oat spelt xylan + H2O
-
Thermothielavioides terrestris Co3Bag1 ?
-
?

Subunits

Subunits Comment Organism
? x * 82000, SDS-PAGE Thermothielavioides terrestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermothielavioides terrestris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
half-life 23 days Thermothielavioides terrestris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
263
-
carboxymethyl cellulose pH 5.5, 85°C Thermothielavioides terrestris
465
-
birchwood xylan pH 5.5, 85°C Thermothielavioides terrestris
479
-
beechwood xylan pH 5.5, 85°C Thermothielavioides terrestris
550
-
oat spelt xylan pH 5.5, 85°C Thermothielavioides terrestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Thermothielavioides terrestris

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10 more than 90% of maximum activity Thermothielavioides terrestris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
beechwood xylan kcat/Km value 1172 mg/ml/s, pH 5.5, 85°C Thermothielavioides terrestris
additional information
-
carboxymethyl cellulose kcat/Km value 39.7 mg/ml/s, pH 5.5, 85°C Thermothielavioides terrestris
additional information
-
birchwood xylan kcat/Km value 847 mg/ml/s, pH 5.5, 85°C Thermothielavioides terrestris
additional information
-
oat spelt xylan kcat/Km value 956 mg/ml/s, pH 5.5, 85°C Thermothielavioides terrestris