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Literature summary for 3.2.1.8 extracted from

  • Bennett, N.A.; Ryan, J.; Biely, P.; Vrsanska, M.; Kremnicky, L.; Macris, B.J.; Kekos, D.; Christakopoulos, P.; Katapodis, P.; Claeyssens, M.; Nerinckx, W.; Ntauma, P.; Bhat, M.K.
    Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Thermomyces lanuginosus ATCC 46882 (1998), Carbohydr. Res., 306, 445-455 .
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermomyces lanuginosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26300
-
PAGE Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
-
-
Thermomyces lanuginosus ATCC 46882
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermomyces lanuginosus

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Thermomyces lanuginosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
889
-
pH 6.0, 50°C Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood 4-O-methyl-D-glucuronoxylan + H2O
-
Thermomyces lanuginosus xylose + xylobiose + ?
-
?
beechwood 4-O-methyl-D-glucuronoxylan + H2O
-
Thermomyces lanuginosus ATCC 46882 xylose + xylobiose + ?
-
?
additional information no substrates: 4-nitrophenyl beta-D--xyloside, 4-nitrophenyl beta-cellobioside Thermomyces lanuginosus ?
-
?
additional information no substrates: 4-nitrophenyl beta-D--xyloside, 4-nitrophenyl beta-cellobioside Thermomyces lanuginosus ATCC 46882 ?
-
?
O-acetyl-4-O-methyl-D-glucuronoxylan + H2O
-
Thermomyces lanuginosus xylose + xylobiose + ?
-
?
O-acetyl-4-O-methyl-D-glucuronoxylan + H2O
-
Thermomyces lanuginosus ATCC 46882 xylose + xylobiose + ?
-
?
rhodymenan + H2O
-
Thermomyces lanuginosus xylose + xylobiose + ?
-
?
rhodymenan + H2O
-
Thermomyces lanuginosus ATCC 46882 xylose + xylobiose + ?
-
?
xylotriose + H2O
-
Thermomyces lanuginosus xylobiose + ?
-
?
xylotriose + H2O
-
Thermomyces lanuginosus ATCC 46882 xylobiose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 27500, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Thermomyces lanuginosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
85
-
more than 50% of maximum activity Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
pH 10, stable for 5 h Thermomyces lanuginosus
60
-
pH 5-9, stable for 5 h Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
Thermomyces lanuginosus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 more than 50% of maximum activity Thermomyces lanuginosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 stable Thermomyces lanuginosus

pI Value

Organism Comment pI Value Maximum pI Value
Thermomyces lanuginosus isoelectric focusing
-
3.7