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Literature summary for 3.2.1.8 extracted from

  • Wang, Y.; Fu, Z.; Huang, H.; Zhang, H.; Yao, B.; Xiong, H.; Turunen, O.
    Improved thermal performance of Thermomyces lanuginosus GH11 xylanase by engineering of an N-terminal disulfide bridge (2012), Biores. Technol., 112, 275-279 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Thermomyces lanuginosus

Protein Variants

Protein Variants Comment Organism
Q1C/Q24C introduction of disulfide bridge, leading to increase in temperature optimum at pH 6.5 by about 10 degrees to 75°C, increased resistance to thermal inactivation and increased melting temperature. At pH 8 and 70°C, the disulfide bridge increases the enzyme half-life 20fold in the presence of substrate Thermomyces lanuginosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermomyces lanuginosus
-
-

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus O43097
-
-
Thermomyces lanuginosus DSM 10635 O43097
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oat xylan + H2O
-
Thermomyces lanuginosus ?
-
?
oat xylan + H2O
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Thermomyces lanuginosus DSM 10635 ?
-
?

Synonyms

Synonyms Comment Organism
XynA
-
Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
wild-type Thermomyces lanuginosus
75
-
mutant Q1C/Q24C Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
66
-
melting temperature, wild-type Thermomyces lanuginosus
70
-
wild-type at pH 8.0, half-life 11 min in presence of substrate, 33 min in absence of substrate, respectively. Mutant Q1C/Q24C at pH 8.0, half-life 229 min in presence of substrate, 315 min in absence of substrate, respectively. Mutant Q1C/Q24C at pH 9.0, half-life 112 min in presence of substrate, 169 min in absence of substrate, respectively Thermomyces lanuginosus
74
-
melting temperature, mutant Q1C/Q24C Thermomyces lanuginosus