Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.8 extracted from

  • Damaso, M.C.; Almeida, M.S.; Kurtenbach, E.; Martins, O.B.; Pereira, N.; Andrade, C.M.; Albano, R.M.
    Optimized expression of a thermostable xylanase from Thermomyces lanuginosus in Pichia pastoris (2003), Appl. Environ. Microbiol., 69, 6064-6072 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis optimization of recombinant enzyme production in 1-liter flasks. Initial cell density is the most important parameter. Under optimized conditions, 1498 mg xylanase per liter can be achieved Thermomyces lanuginosus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Thermomyces lanuginosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermomyces lanuginosus
-
-

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus O43097
-
-

Subunits

Subunits Comment Organism
? x * 21900 or x * 22100 or x * 22300, mass spectrometry of recombinant protein with variability in the amino terminus, x * 26900, SDS-PAGE Thermomyces lanuginosus

Synonyms

Synonyms Comment Organism
XynA
-
Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable for at least 4 h Thermomyces lanuginosus
70
-
80 min, 60% residual activity Thermomyces lanuginosus
80
-
40 min, 60% residual activity Thermomyces lanuginosus