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Literature summary for 3.2.1.8 extracted from
- Residue mutations of xylanase in Aspergillus kawachii alter its optimum pH (2016), Microbiol. Res., 182, 1-7.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Aspergillus luchuensis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Aspergillus luchuensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D48N/T64S | shift in pH optimum from 2.0 to 5.0 | Aspergillus luchuensis |
| T64S | shift in pH optimum from 2.0 to 3.8 | Aspergillus luchuensis |
General Stability
| General Stability | Organism |
|---|---|
| enzyme is strongly resistant to protease digestion. After treatment at 37°C for 30 min with pepsin or trypsin, the enzyme retains more than 90% of the maximum xylanase activity | Aspergillus luchuensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | partial inhibition | Aspergillus luchuensis | |
| Mn2+ | more than 50% inhibition | Aspergillus luchuensis | |
| SDS | more than 50% inhibition | Aspergillus luchuensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
beechwood xylan | mutant T64S, Km value 10 mg/ml, pH 3.8, 45°C | Aspergillus luchuensis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | slight activation | Aspergillus luchuensis | |
| Ca2+ | slight activation | Aspergillus luchuensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
- |
Aspergillus luchuensis |
| 23300 | - |
- |
Aspergillus luchuensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus luchuensis | P33557 | - |
- |
| Aspergillus luchuensis NBRC 4308 | P33557 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 350 | - |
wild-type, pH 3.8, 45°C | Aspergillus luchuensis |
| 585 | - |
mutant T64S, presence of Al3+, pH 3.8, 45°C | Aspergillus luchuensis |
| 603 | - |
mutant T64S, presence of Ca2+, pH 3.8, 45°C | Aspergillus luchuensis |
| 687 | - |
mutant T64S, presence of dithiothreitol, pH 3.8, 45°C | Aspergillus luchuensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beechwood xylan + H2O | - |
Aspergillus luchuensis | ? | - |
? | |
| beechwood xylan + H2O | - |
Aspergillus luchuensis NBRC 4308 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23000, SDS-PAGE, x * 23300, calculated | Aspergillus luchuensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XynC | - |
Aspergillus luchuensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Aspergillus luchuensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
mutant T64S, 30 min, 77% resiudal activity | Aspergillus luchuensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
wild-type | Aspergillus luchuensis |
| 3.8 | - |
mutant T64S | Aspergillus luchuensis |
| 5 | - |
mutant D48N/T64S | Aspergillus luchuensis |
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