Literature summary for 3.2.1.8 extracted from

Freire, F.; Verma, A.; Bule, P.; Alves, V.; Fontes, C.; Goyal, A.; Najmudin, S.
Conservation in the mechanism of glucuronoxylan hydrolysis revealed by the structure of glucuronoxylan xylanohydrolase (CtXyn30A) from Clostridium thermocellum (2016), Acta Crystallogr. Sect. D, 72, 1162-1173.

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure of a truncated derivative of comprising exclusively the enzyme catalytic module. The enzyme displays a (beta/alpha)8 TIM-barrel core with a side-associated beta-sheet domain. In mutant E225A, solved in the presence of xylotetraose, xylotetraose-cleavage oligosaccharides partially occupy subsites -3 to +2. The sugar ring at the +1 subsite is held in place by hydrophobic stacking interactions between Tyr139 and Tyr200 and hydrogen bonds to the OH group of Tyr227. The xylopyranosyl ring at the -1 subsite appears in the alpha-anomeric configuration	Acetivibrio thermocellus

Crystallization (Comment)

Organism

structure of a truncated derivative of comprising exclusively the enzyme catalytic module. The enzyme displays a (beta/alpha)8 TIM-barrel core with a side-associated beta-sheet domain. In mutant E225A, solved in the presence of xylotetraose, xylotetraose-cleavage oligosaccharides partially occupy subsites -3 to +2. The sugar ring at the +1 subsite is held in place by hydrophobic stacking interactions between Tyr139 and Tyr200 and hydrogen bonds to the OH group of Tyr227. The xylopyranosyl ring at the -1 subsite appears in the alpha-anomeric configuration

Acetivibrio thermocellus

Protein Variants

Protein Variants	Comment	Organism
E225A	mutation in catalytic residue, crystallization data	Acetivibrio thermocellus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cellulosome	-	Acetivibrio thermocellus	43263	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	-	Acetivibrio thermocellus

Organism

Organism	UniProt	Comment	Textmining
Acetivibrio thermocellus	A3DJS9	-	-
Acetivibrio thermocellus DSM 1237	A3DJS9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	acts predominantly on polysaccharides with xylan as the main chain and substituted with varying amounts of glucuronic acids, such as in beechwood xylan, birchwood xylan and 4-O-methylglucuronoxylan	Acetivibrio thermocellus	?	-	?
additional information	acts predominantly on polysaccharides with xylan as the main chain and substituted with varying amounts of glucuronic acids, such as in beechwood xylan, birchwood xylan and 4-O-methylglucuronoxylan	Acetivibrio thermocellus DSM 1237	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 45000, calcuated	Acetivibrio thermocellus

Synonyms

Synonyms	Comment	Organism
Cthe_3012	-	Acetivibrio thermocellus
Xyn30A	-	Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	-	Acetivibrio thermocellus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	70	more than 95% of maximum activity	Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Acetivibrio thermocellus