Any feedback?
Literature summary for 3.2.1.8 extracted from
- Production of alkaliphilic, halotolerent, thermostable cellulase free xylanase by Bacillus halodurans PPKS-2 using agro waste: single step purification and characterization (2012), World J. Microbiol. Biotechnol., 28, 183-192.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, 114% of initial activity | Halalkalibacterium halodurans |  |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | yield of enzyme is enhanced more than four fold in the presence of 1% corn husk and 0.5% peptone or feather hydrolysate at pH 11 and 37°C | Halalkalibacterium halodurans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 1 mM, 65% residual activity | Halalkalibacterium halodurans | |
| Hg2+ | 1 mM, 10% residual activity | Halalkalibacterium halodurans | |
| Mn2+ | 1 mM, 4.8% residual activity | Halalkalibacterium halodurans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
oat spelt xylan | Km value is 4.2 mg/ml, pH 11, 70°C | Halalkalibacterium halodurans | |
| additional information | - |
birchwood xylan | Km value is 4.4 mg/ml, pH 11, 70°C | Halalkalibacterium halodurans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Halalkalibacterium halodurans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
x * 24000, SDS-PAGE | Halalkalibacterium halodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halalkalibacterium halodurans | - |
- |
- |
| Halalkalibacterium halodurans PPKS-2 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Halalkalibacterium halodurans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 214 | - |
pH 11, 70°C | Halalkalibacterium halodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| birchwood xylan + H2O | - |
Halalkalibacterium halodurans | D-xylose + ? | - |
? | |
| birchwood xylan + H2O | - |
Halalkalibacterium halodurans PPKS-2 | D-xylose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Halalkalibacterium halodurans | D-xylose + ? | - |
? | |
| oat spelt xylan + H2O | - |
Halalkalibacterium halodurans PPKS-2 | D-xylose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24000, SDS-PAGE | Halalkalibacterium halodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Halalkalibacterium halodurans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
80% of maximum activity | Halalkalibacterium halodurans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
3 h, no loss of activity | Halalkalibacterium halodurans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 11 | - |
- |
Halalkalibacterium halodurans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 10 | 12 | more than 75% of maximum activity within | Halalkalibacterium halodurans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
