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Literature summary for 3.2.1.8 extracted from

  • Deesukon, W.; Nishimura, Y.; Sakamoto, T.; Sukhumsirichart, W.
    Purification, characterization of GH11 endo-beta-1,4-xylanase from thermotolerant Streptomyces sp. SWU10 and overexpression in Pichia pastoris KM71H (2013), Mol. Biotechnol., 54, 37-46.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Pichia pastoris Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
additional information recombinant protein expressed in Pichia pastoris without signal peptide exhibits better physicochemical properties than those of the native enzyme including higher optimal temperature of 60°C, and specific activity, but lower optimal pH 4.0 Streptomyces sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Streptomyces sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
x * 24000, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp. F7J663
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sequence contains six potential glycosylation sites Streptomyces sp.

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.3
-
native enzyme, pH 6.0, 37°C Streptomyces sp.
77.4
-
recombinant enzyme, pH 6.0, 37°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birchwood xylan + H2O
-
Streptomyces sp. ?
-
?
oat spelt xylan + H2O 52% of the activity with birchwood xylan Streptomyces sp. xylobiose + xylotriose + ? major products ?
wheat arabinoxylan + H2O 35% of the activity with birchwood xylan Streptomyces sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 24000, SDS-PAGE Streptomyces sp.

Synonyms

Synonyms Comment Organism
XynSW1
-
Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable up to, for 1 h Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Streptomyces sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 11 4°C, 16 h, more than 80% of initial activity Streptomyces sp.