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Literature summary for 3.2.1.8 extracted from

  • Kim, d.o..Y.; Shin, D.H.; Jung, S.; Kim, H.; Lee, J.S.; Cho, H.Y.; Bae, K.S.; Sung, C.K.; Rhee, Y.H.; Son, K.H.; Park, H.Y.
    Novel alkali-tolerant GH10 endo-beta-1,4-xylanase with broad substrate specificity from Microbacterium trichothecenolyticum HY-17, a gut bacterium of the mole cricket Gryllotalpa orientalis (2014), J. Microbiol. Biotechnol., 24, 943-953.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, no residual activity Microbacterium trichothecenolyticum
N-bromosuccinimide 1 mM, 39.6% residual ativity Microbacterium trichothecenolyticum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 123% of initial activity Microbacterium trichothecenolyticum
Mn2+ 1 mM, 131% of initial activity Microbacterium trichothecenolyticum
Triton X-100 0.5%, 123% of initial activity Microbacterium trichothecenolyticum
Tween 80 0.5%, 121% of initial activity Microbacterium trichothecenolyticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41584
-
x * 41584, calculated Microbacterium trichothecenolyticum

Organism

Organism UniProt Comment Textmining
Microbacterium trichothecenolyticum A0A067XRW0
-
-
Microbacterium trichothecenolyticum HY-17 A0A067XRW0
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53
-
substrate wheat arabinoxylan xylan, pH 9.0, 60°C Microbacterium trichothecenolyticum
75
-
substrate birchwood xylan, pH 9.0, 60°C Microbacterium trichothecenolyticum
83
-
substrate beechwood xylan, pH 9.0, 60°C Microbacterium trichothecenolyticum
97
-
substrate oat spelt xylan, pH 9.0, 60°C Microbacterium trichothecenolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beechwood xylan + H2O
-
Microbacterium trichothecenolyticum xylobiose + xylotriose end products are 71.2% xylobiose + 28.8% xylotriose ?
beechwood xylan + H2O
-
Microbacterium trichothecenolyticum HY-17 xylobiose + xylotriose end products are 71.2% xylobiose + 28.8% xylotriose ?
birchwood xylan + H2O
-
Microbacterium trichothecenolyticum ?
-
?
birchwood xylan + H2O
-
Microbacterium trichothecenolyticum HY-17 ?
-
?
additional information enzyme displays endo-beta-1,4-xylanase activity together with beta-1,3/beta-1,4-glucanase activity. No substrate: xylobiose Microbacterium trichothecenolyticum ?
-
?
additional information enzyme displays endo-beta-1,4-xylanase activity together with beta-1,3/beta-1,4-glucanase activity. No substrate: xylobiose Microbacterium trichothecenolyticum HY-17 ?
-
?
oat spelt xylan + H2O
-
Microbacterium trichothecenolyticum ?
-
?
oat spelt xylan + H2O
-
Microbacterium trichothecenolyticum HY-17 ?
-
?
wheat arabinoxylan + H2O
-
Microbacterium trichothecenolyticum ?
-
?
wheat arabinoxylan + H2O
-
Microbacterium trichothecenolyticum HY-17 ?
-
?

Subunits

Subunits Comment Organism
? x * 41584, calculated Microbacterium trichothecenolyticum

Synonyms

Synonyms Comment Organism
xylH
-
Microbacterium trichothecenolyticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Microbacterium trichothecenolyticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Microbacterium trichothecenolyticum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10
-
Microbacterium trichothecenolyticum

pI Value

Organism Comment pI Value Maximum pI Value
Microbacterium trichothecenolyticum calculated
-
4.8