Cloned (Comment) | Organism |
---|---|
gene xyl1, expression in Pichia pastoris | Acidomyces acidophilus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant XYL1p, hanging drop vapour diffusion method, room temperature, 10 mg/ml protein, from 2 M ammonium sulfate, 100 mM CAPS, pH 10.5, and 200 mM lithium sulfate, X-ray diffraction structure determination and analysis at 1.9 A resolution, modeling | Acidomyces acidophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidomyces acidophilus | Q6VAY1 | gene XYL1 | - |
Purification (Comment) | Organism |
---|---|
recombinant XYL1p from Pichia astoris by anion exchange chromatography | Acidomyces acidophilus |
Subunits | Comment | Organism |
---|---|---|
More | homology modeling, enzyme XYL1p has the overall fold typical to family 11 xylanases, comparison of XYL1 structure with other homologous acidophilic, neutrophilic and alkalophilic xylanases, overview | Acidomyces acidophilus |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-xylanase | - |
Acidomyces acidophilus |
XYL1p | - |
Acidomyces acidophilus |
General Information | Comment | Organism |
---|---|---|
additional information | several sequence and structure modifications appears to be responsible for the acidophilic characteristic of XYL1p 1. The presence of an aspartic acid H bonded to the acid/base catalyst 2. the nature of specifically conserved residues in the active site 3. the negative potential at the surface 4. the decreased number of salt bridges and H bonds in comparison with highly alkaline enzymes | Acidomyces acidophilus |