Cloned (Comment) | Organism |
---|---|
gene xyn11A, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli, expression of the gene with intron, and the gene without intron under the control of AOX1 promoter in Pichia pastoris strain GS115, the enzyme is secreted to the culture medium | Thermochaetoides thermophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermochaetoides thermophila | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | Q8J1V6 | gene xyn11A | - |
Thermochaetoides thermophila NIBGE 1 | Q8J1V6 | gene xyn11A | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Thermochaetoides thermophila | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant xylanase from gene xyn698 with intron gives 15.6 U/ml, that of gene xyn699 without intron results in 1.26 U/ml after 96 h growth | Thermochaetoides thermophila |
Synonyms | Comment | Organism |
---|---|---|
beta-1,4-xylanase | - |
Thermochaetoides thermophila |
xylanase | - |
Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified recombinant enzyme expressed from gene with intron, half-life is 1959 min | Thermochaetoides thermophila |
40 | - |
purified recombinant enzyme expressed from gene with intron, half-life is 959 min | Thermochaetoides thermophila |
45 | - |
purified recombinant enzyme expressed from gene with intron, 10 min, 100% activity remaining | Thermochaetoides thermophila |
50 | - |
purified recombinant enzyme expressed from gene with intron, half-life is 176 min | Thermochaetoides thermophila |
60 | - |
purified recombinant enzyme expressed from gene with intron, half-life is 72 min | Thermochaetoides thermophila |
70 | - |
purified recombinant enzyme expressed from gene with intron, half-life is 39.4 min | Thermochaetoides thermophila |
80 | - |
purified recombinant enzyme expressed from gene with intron, half-life is 18.7 min | Thermochaetoides thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermochaetoides thermophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | purified recombinant enzyme, quite stable | Thermochaetoides thermophila |