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Literature summary for 3.2.1.8 extracted from

  • Chanjuan, L.; Hong, Y.; Shao, Z.; Lin, L.; Huang, X.; Liu, P.; Wu, G.; Meng, X.; Liu, Z.
    Novel alkali-stable, cellulase-free xylanase from deep-sea Kocuria sp. Mn22 (2009), J. Microbiol. Biotechnol., 19, 873-880.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Kxyn, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21 (DE3) Kocuria sp. Mn22

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Kocuria sp. Mn22
Cu2+
-
Kocuria sp. Mn22
Fe2+
-
Kocuria sp. Mn22
Hg2+
-
Kocuria sp. Mn22
Mn2+
-
Kocuria sp. Mn22
additional information Kxyn is not affected by 2-mercaptoethanol, DTT, or SDS Kocuria sp. Mn22
Pb2+
-
Kocuria sp. Mn22
Zn2+
-
Kocuria sp. Mn22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km for beta-1,4-xylan from birchwood is 5.4 mg/ml Kocuria sp. Mn22

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Kxyn is not affected by Ca2+, Mg2+, Na+, K+ Kocuria sp. Mn22

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Kocuria sp. Mn22
-
xylobiose + xylotriose + ? predominant products ?

Organism

Organism UniProt Comment Textmining
Kocuria sp. Mn22 B8XY24 gene Kxyn
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21 (DE3) Kocuria sp. Mn22

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
Kocuria sp. Mn22 xylobiose + xylotriose + ? predominant products ?
beta-1,4-xylan + H2O from birchwood Kocuria sp. Mn22 xylobiose + xylotriose + ? predominant products ?

Synonyms

Synonyms Comment Organism
endo-beta-1,4-xylanase
-
Kocuria sp. Mn22
More the enzyme belongs to the glycosyl hydrolase family 10, GH10 Kocuria sp. Mn22
xylanase
-
Kocuria sp. Mn22

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Kocuria sp. Mn22

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 activity range Kocuria sp. Mn22

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
purified recombinant enzyme, 60 min, 70% activity remaining Kocuria sp. Mn22
60
-
purified recombinant enzyme, 60 min, 30% activity remaining Kocuria sp. Mn22
70
-
purified recombinant enzyme, 60 min, 10% activity remaining Kocuria sp. Mn22

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
185.1
-
beta-1,4-xylan pH 8.5, 55°C, birchwood xylan Kocuria sp. Mn22

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Kocuria sp. Mn22

pH Range

pH Minimum pH Maximum Comment Organism
6 12 activity range Kocuria sp. Mn22

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.6 12 purified recombinant enzyme, over 80% remaining activity after overnight incubation Kocuria sp. Mn22