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Literature summary for 3.2.1.8 extracted from
- Cloning of a Bacillus subtilis AMX-4 xylanase gene and characterization of the gene product (2009), J. Microbiol. Biotechnol., 19, 1514-1519.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene xylA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight inhibition | Bacillus subtilis |  |
| Cu2+ | - |
Bacillus subtilis | |
| EDTA | slight inhibition | Bacillus subtilis | |
| Fe2+ | - |
Bacillus subtilis | |
| Mn2+ | - |
Bacillus subtilis | |
| SDS | slight inhibition | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
x * 23253, sequence calculation, x * 23000, SDS-PAGE | Bacillus subtilis |
| 23253 | - |
x * 23253, sequence calculation, x * 23000, SDS-PAGE | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,4-xylan + H2O | Bacillus subtilis | - |
xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? | |
| beta-1,4-xylan + H2O | Bacillus subtilis AMX-4 | - |
xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
isolated from soil, gene xylA | - |
| Bacillus subtilis AMX-4 | - |
isolated from soil, gene xylA | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arabinoxylan + H2O | - |
Bacillus subtilis | ? | - |
? | |
| arabinoxylan + H2O | - |
Bacillus subtilis AMX-4 | ? | - |
? | |
| beta-1,4-xylan + H2O | - |
Bacillus subtilis | xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? | |
| beta-1,4-xylan + H2O | from oat spelt or birchwood | Bacillus subtilis | xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? | |
| beta-1,4-xylan + H2O | - |
Bacillus subtilis AMX-4 | xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? | |
| beta-1,4-xylan + H2O | from oat spelt or birchwood | Bacillus subtilis AMX-4 | xylobiose + xylotriose + ? | the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose | ? | |
| additional information | AMX-4 xylanase is not active toward other polysaccharides including carboxymethylcellulose, locust bean gum, lichenan, laminarin, and Avicel or toward synthetic substrate derivatives such as 4-nitrophenyl-beta-xylopyranoside, 4-nitrophenyl-beta-mannoside, and 4-nitrophenyl-beta-cellobioside, it can hydrolyze only beta-1,4-xylosidic linkages and has no beta-xylosidase activity | Bacillus subtilis | ? | - |
? | |
| additional information | AMX-4 xylanase is not active toward other polysaccharides including carboxymethylcellulose, locust bean gum, lichenan, laminarin, and Avicel or toward synthetic substrate derivatives such as 4-nitrophenyl-beta-xylopyranoside, 4-nitrophenyl-beta-mannoside, and 4-nitrophenyl-beta-cellobioside, it can hydrolyze only beta-1,4-xylosidic linkages and has no beta-xylosidase activity | Bacillus subtilis AMX-4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23253, sequence calculation, x * 23000, SDS-PAGE | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AMX-4 xylanase | - |
Bacillus subtilis |
| More | the enzyme belongs to glycosyl hydrolase family 11, GH11 | Bacillus subtilis |
| xylanase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Bacillus subtilis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus subtilis | - |
- |
9.27 |
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