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Literature summary for 3.2.1.8 extracted from
- Functional characterisation of a recombinant xylanase from Pichia pastoris and effect of the enzyme on nutrient digestibility in weaned pigs (2010), Br. J. Nutr., 103, 1507-1513.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | unprocessed Xyn2, supplemented to a xylan-containing diet for weaned pigs, results an average body-weight gain increased by 16.9% with added Xyn2 at a concentration of 500 U/kg diet | Trichoderma reesei |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene xyn2, expression in Pichia pastoris strain PX-1 | Trichoderma reesei |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
x * 21000, Xyn2, SDS-PAGE | Trichoderma reesei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma reesei | - |
gene xyn2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant Xyn2 from Pichia pastoris strain PX-1 | Trichoderma reesei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-1,4-xylan + H2O | from oat spelt, birchwood, or beechwood, specificity, overview | Trichoderma reesei | xylotriose + xylobiose + ? | as the main degradation products of Xyn2 | ? |  |
| additional information | Xyn2 shows no activity with gellan gum, Avicel, and carboxymethyl cellulose | Trichoderma reesei | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 21000, Xyn2, SDS-PAGE | Trichoderma reesei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoxylanase | - |
Trichoderma reesei |
| xylanase 2 | - |
Trichoderma reesei |
| Xyn2 | - |
Trichoderma reesei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
Xyn2 | Trichoderma reesei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 90 | activity range, profile, overview | Trichoderma reesei |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified enzyme, 30 min, loss of less that 10% activity | Trichoderma reesei |
| 60 | - |
purified enzyme, 30 min, loss of about 70% activity | Trichoderma reesei |
| 70 | - |
purified enzyme, 30 min, loss of over 90% activity | Trichoderma reesei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 339.6 | - |
beta-1,4-xylan | beechwood xylan, pH 6.0, 60°C, recombinant Xyn2 | Trichoderma reesei | |
| 435.7 | - |
beta-1,4-xylan | birchwood xylan, pH 6.0, 60°C, recombinant Xyn2 | Trichoderma reesei | |
| 512.4 | - |
beta-1,4-xylan | oat spelt xylan, pH 6.0, 60°C, recombinant Xyn2 | Trichoderma reesei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
Xyn2 | Trichoderma reesei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | activity range, profile, overview | Trichoderma reesei |
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