Cloned (Comment) | Organism |
---|---|
gene Xyn2, expression in Pichia pastoris under the control of the methanol inducible alcohol oxidase 1, AOX1, promoter. The recombiannt enzyme is secreted to the culture medium | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Trichoderma reesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
x * 21000, recombinant enzyme, SDS-PAGE | Trichoderma reesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | Trichoderma reesei | - |
? | - |
? | |
beta-1,4-xylan + H2O | Trichoderma reesei RUT C-30 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | B2CNY5 | gene xyn2 | - |
Trichoderma reesei RUT C-30 | B2CNY5 | gene xyn2 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified recombinant enzyme, 281.2 U/ml with birchwood xylan, 211.0 U/ml with beechwood xylan, and 261.0 U/ml with oat spelt xylan | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-xylan + H2O | - |
Trichoderma reesei | ? | - |
? | |
beta-1,4-xylan + H2O | xylan from birchwood, beechwood, and oat spelt | Trichoderma reesei | ? | - |
? | |
beta-1,4-xylan + H2O | - |
Trichoderma reesei RUT C-30 | ? | - |
? | |
beta-1,4-xylan + H2O | xylan from birchwood, beechwood, and oat spelt | Trichoderma reesei RUT C-30 | ? | - |
? | |
additional information | no activity with Avicel, carboxymethylcellulose, and gellan gum | Trichoderma reesei | ? | - |
? | |
additional information | no activity with Avicel, carboxymethylcellulose, and gellan gum | Trichoderma reesei RUT C-30 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21000, recombinant enzyme, SDS-PAGE | Trichoderma reesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Trichoderma reesei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant enzyme, 30 min, 94% activity remaining | Trichoderma reesei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
139.6 | - |
beta-1,4-xylan | pH 5.0, 50°C, beechwood xylan | Trichoderma reesei | |
168.7 | - |
beta-1,4-xylan | pH 5.0, 50°C, oat spelt xylan | Trichoderma reesei | |
205.7 | - |
beta-1,4-xylan | pH 5.0, 50°C, birchwood xylan | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Trichoderma reesei |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | - |
Trichoderma reesei |