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Literature summary for 3.2.1.8 extracted from

  • Arora, N.; Banerjee, A.K.; Mutyala, S.; Murty, U.S.
    Comparative characterization of commercially important xylanase enzymes (2009), Bioinformation, 3, 446-453.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
paper production since XynA is thermostable and has no cellulase activity it can be potentially used for pretreatment of paper pulp before bleaching Paecilomyces sp. 'thermophila'

Cloned(Commentary)

Cloned (Comment) Organism
gene xynA, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Paecilomyces sp. 'thermophila'

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, Km values of XynA for birchwood xylan, beechwood xylan, and oat-spelt xylan are 4.4 mg/ml, 3.6 mg/ml, and 9.7 mg/ml, respectively Paecilomyces sp. 'thermophila'

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26132
-
x * 26132, XynA, sequence calculation, x * 28000, XynA, SDS-PAGE Paecilomyces sp. 'thermophila'
28000
-
x * 26132, XynA, sequence calculation, x * 28000, XynA, SDS-PAGE Paecilomyces sp. 'thermophila'

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Paecilomyces sp. 'thermophila' beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone ?
-
?
additional information Paecilomyces sp. 'thermophila' J18 beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone ?
-
?

Organism

Organism UniProt Comment Textmining
Paecilomyces sp. 'thermophila' D1G4K3 gene XynA
-
Paecilomyces sp. 'thermophila' J18 D1G4K3 gene XynA
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the protein sequence contains one possible N-glycosylation site, consensus Asn-Xaa-Ser/Thr, at position 25 and two potential O-glycosylation sites at positions 26/27, respectively Paecilomyces sp. 'thermophila'

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 48fold from Escherichia coli by nickel affinity chromatography Paecilomyces sp. 'thermophila'

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
706.1
-
purified recombinant XynA Paecilomyces sp. 'thermophila'

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O xylan from birchwood, beechwood, and oat spelt Paecilomyces sp. 'thermophila' ?
-
?
beta-1,4-xylan + H2O xylan from birchwood, beechwood, and oat spelt Paecilomyces sp. 'thermophila' J18 ?
-
?
additional information beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone Paecilomyces sp. 'thermophila' ?
-
?
additional information the enzyme has an endohydrolytic mode of action and can hydrolyse xylotriose to xylobiose through transglycosylation Paecilomyces sp. 'thermophila' ?
-
?
additional information beta-1,4-endoxylanases are enzymes which randomly hydrolyse the beta-1,4-glycosidic bonds in the xylan backbone Paecilomyces sp. 'thermophila' J18 ?
-
?
additional information the enzyme has an endohydrolytic mode of action and can hydrolyse xylotriose to xylobiose through transglycosylation Paecilomyces sp. 'thermophila' J18 ?
-
?

Subunits

Subunits Comment Organism
? x * 26132, XynA, sequence calculation, x * 28000, XynA, SDS-PAGE Paecilomyces sp. 'thermophila'

Synonyms

Synonyms Comment Organism
beta-1,4endoxylanase
-
Paecilomyces sp. 'thermophila'
xylanase
-
Paecilomyces sp. 'thermophila'
XynA
-
Paecilomyces sp. 'thermophila'

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
recombinant His-tagged enzyme Paecilomyces sp. 'thermophila'

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
recombinant His-tagged enzyme, 30 min, over 90% activity remaining Paecilomyces sp. 'thermophila'
70
-
recombinant His-tagged enzyme, half-life is 174.8 min Paecilomyces sp. 'thermophila'
75
-
recombinant His-tagged enzyme, half-life is 137.9 min Paecilomyces sp. 'thermophila'
85
-
recombinant His-tagged enzyme, 30 min, over 60% activity remaining, half-life is 68.2 min Paecilomyces sp. 'thermophila'

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
recombinant His-tagged enzyme Paecilomyces sp. 'thermophila'

pI Value

Organism Comment pI Value Maximum pI Value
Paecilomyces sp. 'thermophila' sequence calculation
-
5.47