Literature summary for 3.2.1.8 extracted from

Zhang, S.; Zhang, K.; Chen, X.; Chu, X.; Sun, F.; Dong, Z.
Five mutations in N-terminus confer thermostability on mesophilic xylanase (2010), Biochem. Biophys. Res. Commun., 395, 200-206.

Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant AnxBs in Escherichia coli strain BL21 (DE3)	Aspergillus niger
expression of wild-type and mutant SlxBs in Escherichia coli strain BL21 (DE3)	Thermobifida fusca
expression of wild-type and mutant SoxBs in Escherichia coli strain BL21 (DE3)	Streptomyces olivaceoviridis

Protein Variants

Protein Variants	Comment	Organism
G23A/G24P/S26T	the mutation increases the enzyme's thermostability	Aspergillus niger
G23A/G24P/S26T	the mutation increases the enzyme's thermostability	Thermobifida fusca
G23A/G24P/S26T	the mutation increases the enzyme's thermostability	Streptomyces olivaceoviridis
additional information	construction of an N-terminal replacement mutant	Aspergillus niger
additional information	construction of an N-terminal replacement mutant	Thermobifida fusca
additional information	substituting of Streptomyces olivaceovirdis SoxB 33 N-terminal amino acid residues with the corresponding residues of the thermophilic xylanase TfxA from Thermomonospora fusca strain ATCC 27730 significantly enhances the enzyme thermostability, overview	Streptomyces olivaceoviridis
T11Y/N12H/N13D/F15Y/F16F	the mutation increases the enzyme's thermostability	Aspergillus niger
T11Y/N12H/N13D/F15Y/F16F	the mutation increases the enzyme's thermostability	Thermobifida fusca
T11Y/N12H/N13D/F15Y/F16F	the mutation increases the enzyme's thermostability	Streptomyces olivaceoviridis
T30E/N32G/S33P	the mutation increases the enzyme's thermostability	Aspergillus niger
T30E/N32G/S33P	the mutation increases the enzyme's thermostability	Thermobifida fusca
T30E/N32G/S33P	the mutation increases the enzyme's thermostability	Streptomyces olivaceoviridis
V3A/I4V/T6S/Q8E	the mutation increases the enzyme's thermostability	Aspergillus niger
V3A/I4V/T6S/Q8E	the mutation increases the enzyme's thermostability	Thermobifida fusca
V3A/I4V/T6S/Q8E	the mutation increases the enzyme's thermostability	Streptomyces olivaceoviridis

Organism

Organism	UniProt	Comment	Textmining
Aspergillus niger	-	-	-
Aspergillus niger UV-11	-	-	-
Streptomyces olivaceoviridis	-	strain ATCC 23630	-
Thermobifida fusca	-	strain ATCC 27730	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activities of wild-type and mutant enzymes, overview	Aspergillus niger
additional information	-	activities of wild-type and mutant enzymes, overview	Thermobifida fusca
additional information	-	activities of wild-type and mutant enzymes, overview	Streptomyces olivaceoviridis
427	-	purified recombinant wild-type SlxB	Thermobifida fusca
801	-	purified recombinant wild-type SoxB	Streptomyces olivaceoviridis
1342	-	purified recombinant wild-type AnxB	Aspergillus niger

Subunits

Subunits	Comment	Organism
More	enzyme N-terminal structure analysis and homology modelling, overview	Aspergillus niger
More	enzyme N-terminal structure analysis and homology modelling, overview	Thermobifida fusca
More	enzyme N-terminal structure analysis and homology modelling, overview	Streptomyces olivaceoviridis

Synonyms

Synonyms	Comment	Organism
AnxB	-	Aspergillus niger
More	the enzyme belongs to the glycosyl hydrolase family 11, GH11	Aspergillus niger
More	the enzyme belongs to the glycosyl hydrolase family 11, GH11	Thermobifida fusca
More	the enzyme belongs to the glycosyl hydrolase family 11, GH11	Streptomyces olivaceoviridis
SlxB	-	Thermobifida fusca
SoxB	-	Streptomyces olivaceoviridis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Aspergillus niger
55	-	-	Thermobifida fusca
65	-	-	Streptomyces olivaceoviridis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Aspergillus niger
6	-	assay at	Thermobifida fusca
6	-	assay at	Streptomyces olivaceoviridis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)