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Literature summary for 3.2.1.8 extracted from

  • Shi, P.; Tian, J.; Yuan, T.; Liu, X.; Huang, H.; Bai, Y.; Yang, P.; Chen, X.; Wu, N.; Yao, B.
    Paenibacillus sp. strain E18 bifunctional xylanase-glucanase with a single catalytic domain (2010), Appl. Environ. Microbiol., 76, 3620-3624.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
XynBE18 expression in Escherichia coli strain Bl21(DE3) Paenibacillus sp. E18

Protein Variants

Protein Variants Comment Organism
E129A/E236A the naturally occuring enzyme mutant XynBE18 shows also beta-1,3-1,4-glucan hydrolase activity, EC 3.2.1.6. Recombinant XynBE18 shows specificity toward oat spelt xylan and birchwood xylan and barley beta-1,3-1,4-glucan and lichenin, no activity with carboxymethylcellulose or Avicel, overview Paenibacillus sp. E18

Inhibitors

Inhibitors Comment Organism Structure
Ag+ strong inhibition Paenibacillus sp. E18
Cu2+
-
 Paenibacillus sp. E18
Fe3+
-
 Paenibacillus sp. E18
Hg2+ strong inhibition Paenibacillus sp. E18
Ni2+
-
 Paenibacillus sp. E18
SDS
-
 Paenibacillus sp. E18
Zn2+
-
 Paenibacillus sp. E18

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no significant effect on the xylanase activity of recombinant XynBE18 by 1-10 mM Na, K, Li, Mg2, Ca2, or EDTA Paenibacillus sp. E18

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Paenibacillus sp. E18 natural mutant XynBE18 can accommodate xylan and beta-1,3-1,4-glucan, but XynE2 is only accessible to xylan, the cleft of XynBE18 containing the active site is larger than that of XynE2 xylose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. E18
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
59.6
-
 recombinant XynBE18, substrate oat spelt xylan Paenibacillus sp. E18
61.57
-
 recombinant XynBE18, substrate birchwood xylan Paenibacillus sp. E18

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O natural mutant XynBE18 can accommodate xylan and beta-1,3-1,4-glucan, but XynE2 is only accessible to xylan, the cleft of XynBE18 containing the active site is larger than that of XynE2 Paenibacillus sp. E18 xylose + ?
-
 ?
beta-1,4-xylan + H2O from birchwood and oat spelt Paenibacillus sp. E18 xylose + ?
-
 ?

Subunits

Subunits Comment Organism
More XynBE18 structure-function relationship, homology modeling and molecular dynamic simulation, overview Paenibacillus sp. E18

Synonyms

Synonyms Comment Organism
XynB18
-
 Paenibacillus sp. E18
XynE2
-
 Paenibacillus sp. E18

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Paenibacillus sp. E18

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 profile, overview Paenibacillus sp. E18

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 assay at Paenibacillus sp. E18

pH Range

pH Minimum pH Maximum Comment Organism
5.5 12 activity range, profile, overview Paenibacillus sp. E18