Literature summary for 3.2.1.8 extracted from

Li, N.; Yang, P.; Wang, Y.; Luo, H.; Meng, K.; Wu, N.; Fan, Y.; Yao, B.
Cloning, expression, and characterization of protease-resistant xylanase from Streptomyces fradiae var. k11 (2008), J. Microbiol. Biotechnol., 18, 410-416.

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Application

Application	Comment	Organism
industry	Xyn10 can be an important candidate for protease-resistant mechanistic research and has potential applications in the food industry, cotton scouring, and improving animal nutrition	Streptomyces fradiae

Cloned(Commentary)

Cloned (Comment)	Organism
into vector pET-22b(+) and expressed in Escherichia coli BL21 (DE3)	Streptomyces fradiae

Inhibitors

Inhibitors	Comment	Organism	Structure
Hg2+	completely inhibits	Streptomyces fradiae
additional information	shows resistance to neutral and alkaline proteases, enzyme activity is unchanged after incubation for 2 h with trypsin, chymotrypsin, collagenase, subtilisin A, proteinase K, and proleather, and retains over 60% of its activity following treatment with high concentrations of an alkaline protease from Bacillus pumilus	Streptomyces fradiae
SDS	strongly inhibits by 94.4%	Streptomyces fradiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	enhances activity by 61.9%	Streptomyces fradiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47000	-	1 * 47000, SDS-PAGE	Streptomyces fradiae

Organism

Organism	UniProt	Comment	Textmining
Streptomyces fradiae	A7TVD4	var. K11	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity by acetone precipitation and anion-exchange chromatography, 6.7fold with a final activity yield of 12.7%	Streptomyces fradiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
45.7	-	culture supernatant	Streptomyces fradiae
304.2	-	6.7fold purified enzyme	Streptomyces fradiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
birchwood xylan + H2O	-	Streptomyces fradiae	?	-	?
oat spelt xylan + H2O	-	Streptomyces fradiae	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 47000, SDS-PAGE	Streptomyces fradiae

Synonyms

Synonyms	Comment	Organism
xylanase	-	Streptomyces fradiae
Xyn10	-	Streptomyces fradiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	at pH 7.8	Streptomyces fradiae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	60	remains stable at 50°C after incubation for 30 min at pH 7.8. More than 40% of the activity is retained after treating the enzyme at 60°C for 5 min	Streptomyces fradiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
103.4	-	birchwood xylan	-	Streptomyces fradiae
170.8	-	oat spelt xylan	-	Streptomyces fradiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	at 50°C	Streptomyces fradiae

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8.5	over 80% of the peak activity	Streptomyces fradiae

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	10	after incubation at 37°C for 1 h	Streptomyces fradiae

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Release 2023.1 (January 2023)