Application | Comment | Organism |
---|---|---|
industry | Xyn10 can be an important candidate for protease-resistant mechanistic research and has potential applications in the food industry, cotton scouring, and improving animal nutrition | Streptomyces fradiae |
Cloned (Comment) | Organism |
---|---|
into vector pET-22b(+) and expressed in Escherichia coli BL21 (DE3) | Streptomyces fradiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | completely inhibits | Streptomyces fradiae | |
additional information | shows resistance to neutral and alkaline proteases, enzyme activity is unchanged after incubation for 2 h with trypsin, chymotrypsin, collagenase, subtilisin A, proteinase K, and proleather, and retains over 60% of its activity following treatment with high concentrations of an alkaline protease from Bacillus pumilus | Streptomyces fradiae | |
SDS | strongly inhibits by 94.4% | Streptomyces fradiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | enhances activity by 61.9% | Streptomyces fradiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
1 * 47000, SDS-PAGE | Streptomyces fradiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces fradiae | A7TVD4 | var. K11 | - |
Purification (Comment) | Organism |
---|---|
to homogeneity by acetone precipitation and anion-exchange chromatography, 6.7fold with a final activity yield of 12.7% | Streptomyces fradiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
45.7 | - |
culture supernatant | Streptomyces fradiae |
304.2 | - |
6.7fold purified enzyme | Streptomyces fradiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birchwood xylan + H2O | - |
Streptomyces fradiae | ? | - |
? | |
oat spelt xylan + H2O | - |
Streptomyces fradiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, SDS-PAGE | Streptomyces fradiae |
Synonyms | Comment | Organism |
---|---|---|
xylanase | - |
Streptomyces fradiae |
Xyn10 | - |
Streptomyces fradiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
at pH 7.8 | Streptomyces fradiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | remains stable at 50°C after incubation for 30 min at pH 7.8. More than 40% of the activity is retained after treating the enzyme at 60°C for 5 min | Streptomyces fradiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
103.4 | - |
birchwood xylan | - |
Streptomyces fradiae | |
170.8 | - |
oat spelt xylan | - |
Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
at 50°C | Streptomyces fradiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | over 80% of the peak activity | Streptomyces fradiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | after incubation at 37°C for 1 h | Streptomyces fradiae |