Literature summary for 3.2.1.8 extracted from

Suzuki, R.; Fujimoto, Z.; Ito, S.; Kawahara, S.I.; Kaneko, S.; Taira, K.; Hasegawa, T.; Kuno, A.
Crystallographic snapshots of an entire reaction cycle for a retaining xylanase from Streptomyces olivaceoviridis E-86 (2009), J. Biochem., 146, 61-70.

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Cloned(Commentary)

Cloned (Comment)	Organism
mutants overexpressed in Escherichia coli	Streptomyces olivaceoviridis

Crystallization (Commentary)

Crystallization (Comment)	Organism
switching enzyme with azide, by the hanging-drop vapor-diffusion method, at 1.7 A resolution	Streptomyces olivaceoviridis

Protein Variants

Protein Variants	Comment	Organism
E128H	inhibits the breakdown of the glycosyl-enzyme intermediate. Restoration of the breakdown activity of the mutant by adding exogenous nucleophiles, such as sodium azide, results in a mutant that acts as a switching enzyme with azide	Streptomyces olivaceoviridis
additional information	obtained switching mutant N127S-E128H, rate of hydrolysis is apparently zero in the absence of sodium azide, accumulates the glycosyl-enzyme intermediate, breakdown rate of glycosyl-enzyme intermediate is dramatically accelerated by two orders of magnitude in the presence of 300 mM sodium azide	Streptomyces olivaceoviridis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00789	-	p-nitrophenyl-beta-D-xylobioside	switching enzyme with azide	Streptomyces olivaceoviridis
0.00821	-	p-nitrophenyl-beta-D-xylobioside	mutant E128H	Streptomyces olivaceoviridis
2.37	-	p-nitrophenyl-beta-D-xylobioside	wild-type	Streptomyces olivaceoviridis

Organism

Organism	UniProt	Comment	Textmining
Streptomyces olivaceoviridis	-	-	-
Streptomyces olivaceoviridis E-86	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
mutants purified by one-step affinity purification	Streptomyces olivaceoviridis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-nitrophenyl-beta-D-xylobioside + H2O	-	Streptomyces olivaceoviridis	?	-	?
p-nitrophenyl-beta-D-xylobioside + H2O	-	Streptomyces olivaceoviridis E-86	?	-	?

Synonyms

Synonyms	Comment	Organism
xylanase	-	Streptomyces olivaceoviridis
Xyn10A	-	Streptomyces olivaceoviridis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.005167	-	p-nitrophenyl-beta-D-xylobioside	mutant E128H	Streptomyces olivaceoviridis
0.0523	-	p-nitrophenyl-beta-D-xylobioside	switching enzyme with azide	Streptomyces olivaceoviridis
4.67	-	p-nitrophenyl-beta-D-xylobioside	mutant N127S	Streptomyces olivaceoviridis
42.17	-	p-nitrophenyl-beta-D-xylobioside	wild-type	Streptomyces olivaceoviridis

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Release 2023.1 (January 2023)