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Literature summary for 3.2.1.8 extracted from

  • Hernandez, A.; Lopez, J.C.; Santamaria, R.; Diaz, M.; Fernandez-Abalos, J.M.; Copa-Patino, J.L.; Soliveri, J.
    Xylan-binding xylanase Xyl30 from Streptomyces avermitilis: cloning, characterization, and overproduction in solid-state fermentation (2008), Int. Microbiol., 11, 133-141.
    View publication on PubMed
Search for more literature for 3.2.1.8

Application

Application Comment Organism
industry high production yields of the modular xylanase Xyl30 by solid-state fermentation and its biochemical features make it a good candidate for use in industrial applications Streptomyces avermitilis

Cloned(Commentary)

Cloned (Comment) Organism
3588-bp BamHI-XbaI fragment containing genes xyl30 and hd30 introduced into the pN702GEM3 plasmid and overexpressed in Streptomyces lividans strain 66 Streptomyces avermitilis

Inhibitors

Inhibitors Comment Organism Structure
additional information modular endoxylanase containing a xylan-binding domain, which after processing in the culture supernatant loses the afore mentioned domain and thus its capacity to bind xylan. Sharp decrease (up to 50%) in activity after 15 min of incubation at 70 or 80°C Streptomyces avermitilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later Streptomyces avermitilis
42800
-
 1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later Streptomyces avermitilis
47000
-
 sequence analysis, xyl30 ORF Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis Q9X584
-
-
Streptomyces avermitilis CECT 3339 Q9X584
-
-
Streptomyces lividans
-
-
-
Streptomyces lividans 66
-
-
-
Thermomyces lanuginosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heavier protein band (42.8 kDa) partially purified Streptomyces avermitilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arabinoxylan + H2O
-
 Thermomyces lanuginosus ?
-
 ?
arabinoxylan + H2O highest production of xylanase activity after approximately 3 days of incubation. Only the heavier protein band (42.8 kDa) binds to the substrate Streptomyces avermitilis ?
-
 ?
arabinoxylan + H2O highest production of xylanase activity after approximately 3 days of incubation. Only the heavier protein band (42.8 kDa) binds to the substrate Streptomyces avermitilis CECT 3339 ?
-
 ?
rye bran + H2O low activity with Streptomyces lividans carrying empty vector pN702GEM3 Streptomyces lividans ?
-
 ?
rye bran + H2O maximum production of xylanase occurs after 144 h of incubation. When waterwashed rye bran is used as substrate, maximum production is reached after 216 h Streptomyces avermitilis ?
-
 ?
rye bran + H2O maximum production of xylanase occurs after 144 h of incubation. When waterwashed rye bran is used as substrate, maximum production is reached after 216 h Streptomyces avermitilis CECT 3339 ?
-
 ?
rye bran + H2O low activity with Streptomyces lividans carrying empty vector pN702GEM3 Streptomyces lividans 66 ?
-
 ?
wheat bran + H2O low activity with strain Streptomyces lividans carrying empty vector pN702GEM3 Streptomyces lividans ?
-
 ?
wheat bran + H2O when waterwashed wheat bran is used as substrate, maximum production is reached after 216 h Streptomyces avermitilis ?
-
 ?
wheat bran + H2O when waterwashed wheat bran is used as substrate, maximum production is reached after 216 h Streptomyces avermitilis CECT 3339 ?
-
 ?
wheat bran + H2O low activity with strain Streptomyces lividans carrying empty vector pN702GEM3 Streptomyces lividans 66 ?
-
 ?

Subunits

Subunits Comment Organism
? 1 * 42800, SDS-PAGE, 1 * 35000, SDS-PAGE. Heavier protein band (42.8 kDa) is observed after 12 h of culture, while the lighter band (35 kDa) appears 12 h later Streptomyces avermitilis

Synonyms

Synonyms Comment Organism
endoxylanase
-
 Streptomyces lividans
endoxylanase
-
 Thermomyces lanuginosus
endoxylanase
-
 Streptomyces avermitilis
Xyl30
-
 Streptomyces avermitilis
xylanase
-
 Streptomyces lividans
xylanase
-
 Thermomyces lanuginosus
xylanase
-
 Streptomyces avermitilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 purified heavier protein band (42.8 kDa) Streptomyces avermitilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 purified heavier protein band (42.8 kDa), more than 50% of maximum activity Streptomyces avermitilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 purified heavier protein band (42.8 kDa), retains more than 40% after a 1 h incubation at 70°C. Retains 35% of its activity after a 1 h incubation at 87°C Streptomyces avermitilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 purified heavier protein band (42.8 kDa) Streptomyces avermitilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 purified heavier protein band (42.8 kDa), retains more than 50% of its activity Streptomyces avermitilis

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces avermitilis
-
-
 6.3