Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
S100C/N150C | increased thermostability of about 5°C compared to the wild type enzyme | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus No. 236 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA agarose beads | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35.44 | - |
purified mutant enzyme S100C/N150C | Geobacillus stearothermophilus |
40.58 | - |
purified wild type enzyme | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birchwood xylan + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
birchwood xylan + H2O | - |
Geobacillus stearothermophilus No. 236 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
xylanase | - |
Geobacillus stearothermophilus |
XynA | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus stearothermophilus |