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Literature summary for 3.2.1.8 extracted from

  • Heck, J.X.; Soares, L.H.; Hertz, P.F.; Ayub, M.A.
    Purification and properties of a xylanase produced by Bacillus circulans BL53 on solid-state cultivation (2006), Biochem. Eng. J., 32, 179-184.
No PubMed abstract available
Search for more literature for 3.2.1.8

Activating Compound

Activating Compound Comment Organism Structure
beta-mercaptoethanol 52.66% increase of activity at 2 mM Niallia circulans
dithiothreitol 11.6% increase of activity at 2 mM Niallia circulans
DMSO 6.58% increase of activity at 2 mM Niallia circulans
EDTA 14.48% increase of activity at 2 mM Niallia circulans

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 95.39% residual activity at 2 mM Niallia circulans
Cu2+ 91.44% residual activity at 2 mM Niallia circulans
Fe2+ 81.9% residual activity at 2 mM Niallia circulans
Hg2+ no activity at 2 mM Niallia circulans
Mg2+ 82.55% residual activity at 2 mM Niallia circulans
Na+ 92.1% residual activity at 2 mM Niallia circulans
Pb2+ 78.93% residual activity at 2 mM Niallia circulans
polyhexamethylbiguanid 77.29% residual activity at 2 mM Niallia circulans
SDS 37.46% residual activity at 2 mM Niallia circulans
Zn2+ 91.11% residual activity at 2 mM Niallia circulans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 51.67% increase of activity at 2 mM Niallia circulans
Mn2+ 61.88% increase of activity at 2 mM Niallia circulans
NH4+ 9.21% increase of activity at 2 mM Niallia circulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 SDS-PAGE Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-
Niallia circulans BL53
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate saturation, CM-Sepharose FF column chromatography, and Sephacryl S-200 HR gel filtration Niallia circulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.087
-
 crude extract, using birchwood xylan in 100 mM sodium acetate buffer pH 5.0 as the substrate Niallia circulans
37.22
-
 after 427.83fold purification, using birchwood xylan in 100 mM sodium acetate buffer pH 5.0 as the substrate Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
birchwood xylan + H2O
-
 Niallia circulans D-xylose + ?
-
 ?
birchwood xylan + H2O
-
 Niallia circulans BL53 D-xylose + ?
-
 ?
grape skin + H2O
-
 Niallia circulans ?
-
 ?
grape skin + H2O
-
 Niallia circulans BL53 ?
-
 ?
rice straw + H2O
-
 Niallia circulans ?
-
 ?
rice straw + H2O
-
 Niallia circulans BL53 ?
-
 ?
soybean fiber + H2O
-
 Niallia circulans ?
-
 ?
soybean fiber + H2O
-
 Niallia circulans BL53 ?
-
 ?
soybean hull + H2O
-
 Niallia circulans ?
-
 ?
soybean hull + H2O
-
 Niallia circulans BL53 ?
-
 ?
sugar cane bagasse + H2O
-
 Niallia circulans ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Niallia circulans

Synonyms

Synonyms Comment Organism
1,4-beta-xylan xylanohydrolase
-
 Niallia circulans
xylanase
-
 Niallia circulans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80
-
 Niallia circulans

pH Range

pH Minimum pH Maximum Comment Organism
5 8
-
 Niallia circulans

pI Value

Organism Comment pI Value Maximum pI Value
Niallia circulans isoelectric focusing
-
 8.8