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Literature summary for 3.2.1.8 extracted from

  • Pell, G.; Szabo, L.; Charnock, S.J.; Xie, H.; Gloster, T.M.; Davies, G.J.; Gilbert, H.J.
    Structural and biochemical analysis of Cellvibrio japonicus xylanase 10C: how variation in substrate-binding cleft influences the catalytic profile of family GH-10 xylanases (2004), J. Biol. Chem., 279, 11777-11788.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Cellvibrio japonicus

Protein Variants

Protein Variants Comment Organism
F181Y isoform xylanase 10A, enzymic activity similar to wild-type Cellvibrio japonicus
G295E isoform xylanase 10C, enzymic activity similar to wild-type Cellvibrio japonicus
Y340A isoform xylanase 10C, enzymic activity reduced by more than 90% Cellvibrio japonicus
Y434F isoform xylanase 10C, enzymic activity similar to wild-type Cellvibrio japonicus
Y87A isoform xylanase 10A, reduced enzymic activity Cellvibrio japonicus

Organism

Organism UniProt Comment Textmining
Cellvibrio japonicus
-
isoforms xylanase 10c, xylanase 10A
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 4-O-beta-D-xylopyranosyl-beta-D-xylopyranoside + H2O
-
Cellvibrio japonicus 4-nitrophenol + 4-O-beta-D-xylopyranosyl-beta-D-xylopyranose
-
?
4-nitrophenyl beta-D-cellobioside + H2O
-
Cellvibrio japonicus 4-nitrophenol + beta-D-cellobiose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Cellvibrio japonicus 4-nitrophenol + beta-D-xylopyranose
-
?
arabinoxylan + H2O from wheat or rye Cellvibrio japonicus ?
-
?
glucuronoxylan + H2O
-
Cellvibrio japonicus ?
-
?
additional information very low activity against xylooligosaccharides Cellvibrio japonicus ?
-
?
xylan + H2O from oatspelt Cellvibrio japonicus ?
-
?
xylohexaose + H2O
-
Cellvibrio japonicus ?
-
?
xylotetraose + H2O
-
Cellvibrio japonicus ?
-
?

Subunits

Subunits Comment Organism
More bi-modular enzyme comprising a GH10 catalytic module and a family 15 carbohydrate-binding module, crystallization data Cellvibrio japonicus

Synonyms

Synonyms Comment Organism
xylanase 10A
-
Cellvibrio japonicus
xylanase 10C
-
Cellvibrio japonicus