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Literature summary for 3.2.1.8 extracted from
- Isolation and characterization of the Pseudobutyrivibrio xylanivorans Mz5T xylanase XynT - the first family 11 endoxylanase from rumen Butyrivibrio-related bacteria (2004), Enzyme Microb. Technol., 34, 219-227.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | possible usage as feed additive for animals in order to diminish health problems and enhance proliferation of beneficial microflora | Pseudobutyrivibrio xylanivorans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| partial DNA and amino acid sequencing | Pseudobutyrivibrio xylanivorans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
separation of 2 isozymes with different MW by native PAGE | Pseudobutyrivibrio xylanivorans |
| 30000 | - |
x * 30000, SDS-PAGE | Pseudobutyrivibrio xylanivorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudobutyrivibrio xylanivorans | - |
rumen bacteria, strain Mz5T, DSM14809, enzyme XynT, 2 isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ammonium sulfate precipitation and hydrophobic interaction chromatography, to homogeneity, 2 isozymes | Pseudobutyrivibrio xylanivorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.5 | - |
purified enzyme | Pseudobutyrivibrio xylanivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity against other polysaccharides, no arabinofuranosidase and beta-xylosidase activity, enzyme contains no xylan-binding domain | Pseudobutyrivibrio xylanivorans | ? | - |
? |  |
| xylan + H2O | substrate from brichwood, beechwood, or oat spelt soluble fraction, the latter being the best substrate, endoxylanase activity | Pseudobutyrivibrio xylanivorans | xylooligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Pseudobutyrivibrio xylanivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoxylanase | - |
Pseudobutyrivibrio xylanivorans |
| More | enzyme belongs to family 11 of glycosyl hydrolases | Pseudobutyrivibrio xylanivorans |
| XynT | - |
Pseudobutyrivibrio xylanivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38 | - |
- |
Pseudobutyrivibrio xylanivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
- |
Pseudobutyrivibrio xylanivorans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudobutyrivibrio xylanivorans | purified isozymes with 2 pI values, isoelectric focusing | - |
4.9 |
| Pseudobutyrivibrio xylanivorans | purified isozyme with 2 pI values, isoelectric focusing | - |
6 |
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