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Literature summary for 3.2.1.8 extracted from

  • Martinez-Trujillo, A.; Perez-Avalos, O.; Ponce-Noyola, T.
    Enzymatic properties of a purified xylanase from mutant PN-120 of Cellulomonas flavigena (2003), Enzyme Microb. Technol., 32, 401-406.
No PubMed abstract available
Search for more literature for 3.2.1.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information wild-type and mutant strain enzyme Cellulomonas flavigena

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Cellulomonas flavigena
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, wild-type and mutant strain enzyme, SDS-PAGE Cellulomonas flavigena

Organism

Organism UniProt Comment Textmining
Cellulomonas flavigena
-
 and its mutant PN-120
-
Cellulomonas flavigena CDBB531
-
 and its mutant PN-120
-

Purification (Commentary)

Purification (Comment) Organism
wild-type, 27.5fold, and mutant strain enzyme, 30.6fold, to homogeneity from culture supernatant Cellulomonas flavigena

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
322
-
 purified wild-type enzyme Cellulomonas flavigena
2500
-
 purified mutant enzyme Cellulomonas flavigena

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylan + H2O from birchwood Cellulomonas flavigena ?
-
 ?
xylan + H2O from birchwood Cellulomonas flavigena CDBB531 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, wild-type and mutant strain enzyme, SDS-PAGE Cellulomonas flavigena

Synonyms

Synonyms Comment Organism
xylanase
-
 Cellulomonas flavigena

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55 wild-type enzyme Cellulomonas flavigena
55
-
 mutant enzyme Cellulomonas flavigena

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 wild-type enzyme Cellulomonas flavigena
7
-
 mutant enzyme Cellulomonas flavigena

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 the mutant enzyme shows higher activity at pH 9.0 than the wild-type enzyme Cellulomonas flavigena

pI Value

Organism Comment pI Value Maximum pI Value
Cellulomonas flavigena isoelectric focusing, wild-type and mutant strain enzyme
-
 6