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Literature summary for 3.2.1.8 extracted from

  • Ramos, C.; Morais, H.; Forgacs, E.; Cserhati, T.; Matos, N.; Oliveira, J.S.
    Biochemical characterization of xylanases of four strains of Lentinus edodes (2000), Chem. Environ. Res., 9, 219-227.
No PubMed abstract available
Search for more literature for 3.2.1.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Lentinula edodes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000, Xyl16-3, SDS-PAGE, x * 53000, Xylam, SDS-PAGE, x * 65000, Xyl1-3, SDS-PAGE, x * 90000, Xyl6-6, SDS-PAGE Lentinula edodes
53000
-
 x * 24000, Xyl16-3, SDS-PAGE, x * 53000, Xylam, SDS-PAGE, x * 65000, Xyl1-3, SDS-PAGE, x * 90000, Xyl6-6, SDS-PAGE Lentinula edodes
65000
-
 x * 24000, Xyl16-3, SDS-PAGE, x * 53000, Xylam, SDS-PAGE, x * 65000, Xyl1-3, SDS-PAGE, x * 90000, Xyl6-6, SDS-PAGE Lentinula edodes
90000
-
 x * 24000, Xyl16-3, SDS-PAGE, x * 53000, Xylam, SDS-PAGE, x * 65000, Xyl1-3, SDS-PAGE, x * 90000, Xyl6-6, SDS-PAGE Lentinula edodes

Organism

Organism UniProt Comment Textmining
Lentinula edodes
-
 4 strains
-

Purification (Commentary)

Purification (Comment) Organism
from 4 strains, to homogeneity Lentinula edodes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Lentinula edodes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylan + H2O from birchwood Lentinula edodes ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, Xyl16-3, SDS-PAGE, x * 53000, Xylam, SDS-PAGE, x * 65000, Xyl1-3, SDS-PAGE, x * 90000, Xyl6-6, SDS-PAGE Lentinula edodes

Synonyms

Synonyms Comment Organism
beta-xylanase
-
 Lentinula edodes
Xyl
-
 Lentinula edodes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 Xylam and Xyl6-6, pH 5.0 Lentinula edodes
60
-
 Xyl1-3, pH 5.0 Lentinula edodes
90
-
 Xyl16-3, pH 5.0 Lentinula edodes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 1 h, purified Xyl16-3, 50% remaining activity Lentinula edodes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 Xylam and Xyl16-3 Lentinula edodes
5
-
 Xyl6-6 and Xyl1-3 Lentinula edodes

pI Value

Organism Comment pI Value Maximum pI Value
Lentinula edodes Xyl1-3, isoelectric focusing
-
 6
Lentinula edodes Xyl16-3, isoelectric focusing
-
 6
Lentinula edodes Xyl6-6, isoelectric focusing
-
 7
Lentinula edodes Xylam, isoelectric focusing
-
 7.1