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Literature summary for 3.2.1.8 extracted from
- Biochemical and catalytic properties of an endoxylanase purified from the culture filtrate of Sporotrichum thermophile (2003), Carbohydr. Res., 338, 1881-1890.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (S)-4,5-epoxypentyl-beta-D-xyloside | - |
Thermothelomyces heterothallicus |  |
| omega-epoxyalkyl glycosides of D-xylopyranose | irreversible | Thermothelomyces heterothallicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
4-methylumbelliferyl-beta-xylobiose | pH 5.0, 50°C | Thermothelomyces heterothallicus | |
| 0.5 | - |
2-chloro-4-nitrophenyl beta-D-xylobioside | pH 5.0, 50°C | Thermothelomyces heterothallicus | |
| 0.53 | - |
4-methylumbelliferyl-beta-xylotriose | pH 5.0, 50°C | Thermothelomyces heterothallicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | secretion | Thermothelomyces heterothallicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
x * 25000, SDS-PAGE | Thermothelomyces heterothallicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermothelomyces heterothallicus | - |
strain ATCC 34628 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 13.3fold from culture filtrate to homogeneity by ion exchange and gel filtration chromatography | Thermothelomyces heterothallicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m | mode of action, substrate specificity, substrate binding mechanism | Thermothelomyces heterothallicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 875 | - |
purified enzyme | Thermothelomyces heterothallicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-chloro-4-nitrophenyl beta-D-xylobioside + H2O | - |
Thermothelomyces heterothallicus | ? | - |
? | |
| 4-methylumbelliferyl-beta-xylobiose + H2O | enzyme cleaves at the second glycosidic bond adjacent to the aglycon | Thermothelomyces heterothallicus | ? | - |
? | |
| 4-methylumbelliferyl-beta-xylotriose + H2O | highly preferred substrate, enzyme cleaves at the second glycosidic bond adjacent to the aglycon | Thermothelomyces heterothallicus | ? | - |
? | |
| 4-O-methyl-D-glucuronoxylan + H2O | from beechwood | Thermothelomyces heterothallicus | xylose + xylobiose + ? | main products, also release of acidic xylooligosaccharides | ? | |
| additional information | no activity with 4-nitrophenyl beta-D-xylopyranoside and 4-nitrophenyl cellobioside | Thermothelomyces heterothallicus | ? | - |
? | |
| O-acetyl-4-O-methyl-D-glucuronoxylan + H2O | from beechwood | Thermothelomyces heterothallicus | xylose + xylobiose | main products | ? | |
| rhodymenan + H2O | from beechwood, a beta(1-4)-beta(1-3)-xylan | Thermothelomyces heterothallicus | xylose + xylobiose + xylotetraose + xylopentaose | main products, isomeric oligosaccharides | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, SDS-PAGE | Thermothelomyces heterothallicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoxylanase | - |
Thermothelomyces heterothallicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermothelomyces heterothallicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 5.0, 24 h, 80% remaining activity | Thermothelomyces heterothallicus |
| 60 | - |
half-life 150 min | Thermothelomyces heterothallicus |
| 70 | - |
half-life 15 min | Thermothelomyces heterothallicus |
| 80 | - |
24% remaining activity | Thermothelomyces heterothallicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
4-methylumbelliferyl-beta-xylobiose | pH 5.0, 50°C | Thermothelomyces heterothallicus | |
| 0.13 | - |
2-chloro-4-nitrophenyl beta-D-xylobioside | pH 5.0, 50°C | Thermothelomyces heterothallicus | |
| 66.58 | - |
4-methylumbelliferyl-beta-xylotriose | pH 5.0, 50°C | Thermothelomyces heterothallicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Thermothelomyces heterothallicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
63% remaining activity | Thermothelomyces heterothallicus |
| 5 | - |
50°C, 24 h, 80% remaining activity | Thermothelomyces heterothallicus |
| 6 | - |
55% remaining activity | Thermothelomyces heterothallicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ki values of omega-epoxyalkyl glycosides of D-xylopyranose, overview | Thermothelomyces heterothallicus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermothelomyces heterothallicus | isoelectric focusing | - |
7.1 |
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