Application | Comment | Organism |
---|---|---|
paper production | use in treatment of paper pulps | Thermomyces lanuginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Thermomyces lanuginosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermomyces lanuginosus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
wild-type enzyme, gel filtration | Thermomyces lanuginosus |
24000 | - |
mutant enzyme, gel filtration | Thermomyces lanuginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | - |
strain ATCC 44008, cellulase-free wild-type R-MB and mutant M1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 0.74% carbohydrate content of the wild-type enzyme, 11.8% of the mutant enzyme | Thermomyces lanuginosus |
Purification (Comment) | Organism |
---|---|
native wild-type, 17fold, and mutant enzymes, to homogeneity by anion exchange chromatography and gel filtration | Thermomyces lanuginosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m | histidine, tryptophan residues and carboxylic groups are involved in substrate binding or catalysis | Thermomyces lanuginosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | organism is grown on corn cobs | Thermomyces lanuginosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1660 | - |
purified wild-type enzyme | Thermomyces lanuginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-methyl-glucuronoxylan + H2O | - |
Thermomyces lanuginosus | ? | - |
? | |
additional information | no activity with mannan, araban, galactomannan, inulin, Avicel, soluble starch, sodium carboxymethylcellulose, 2- or 4-nitrophenyl-gluco-, xylo-, manno-, galacto-pyranosides, and arabinofuranoside | Thermomyces lanuginosus | ? | - |
? | |
xylan + H2O | commercial substrate and from oat spelts and larch sawdust, the latter being the best substrate for wild-type and mutant enzyme | Thermomyces lanuginosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 22000, wild-type enzyme, SDS-PAGE, 1 * 24000, mutant enzyme, SDS-PAGE | Thermomyces lanuginosus |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to family 11 of glycosyl hydrolases | Thermomyces lanuginosus |
xylanase | - |
Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
wild-type enzyme | Thermomyces lanuginosus |
70 | - |
mutant enzyme | Thermomyces lanuginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
mutant enzyme retains over 50% activity after 2 h | Thermomyces lanuginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
mutant enzyme | Thermomyces lanuginosus |
7 | - |
wild-type enzyme | Thermomyces lanuginosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | - |
Thermomyces lanuginosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
wild-type and mutant enzymes, stable up to 24 h at 50°C, 60% remaining activity | Thermomyces lanuginosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermomyces lanuginosus | mutant enzyme | - |
6 |
Thermomyces lanuginosus | wild-type enzyme | - |
6 |