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Literature summary for 3.2.1.8 extracted from
- Purification and partial characterization of a basic xylanase produced by thermoalkaliphilic Bacillus sp. strain TAR-1 (2000), Biosci. Biotechnol. Biochem., 64, 887-890.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
x * 23000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
enzyme XynT | - |
| Bacillus sp. (in: Bacteria) TAR-1 | - |
enzyme XynT | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from culture supernatant, to homogeneity | Bacillus sp. (in: Bacteria) |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 270 | - |
purified enzyme | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylan + H2O | from oat spelt | Bacillus sp. (in: Bacteria) | xylobiose + xylotriose + xylotetraose + xylooligosaccharides | - |
? |  |
| xylan + H2O | from oat spelt | Bacillus sp. (in: Bacteria) TAR-1 | xylobiose + xylotriose + xylotetraose + xylooligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| basic xylanase | - |
Bacillus sp. (in: Bacteria) |
| More | enzyme belongs to the family 11/G of endoxylanases | Bacillus sp. (in: Bacteria) |
| XynT | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
sharp decline in activity above at pH 6.0 | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | broad pH profile | Bacillus sp. (in: Bacteria) |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | stable for 3 h at 50°C | Bacillus sp. (in: Bacteria) |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | above, isoelectric focusing | 4.7 | 4.6 |
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