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Literature summary for 3.2.1.8 extracted from
- Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6 (1993), Appl. Environ. Microbiol., 59, 1725-1730.
Application
| Application | Comment | Organism |
|---|---|---|
| paper production | bleaching of pulp | Geobacillus stearothermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 52% inhibition | Geobacillus stearothermophilus |  |
| Ag2+ | 1 mM Ag(NO3)2, 33% inhibition | Geobacillus stearothermophilus | |
| Al3+ | 1 mM, 46% inhibition | Geobacillus stearothermophilus | |
| Cd2+ | - |
Geobacillus stearothermophilus | |
| Co2+ | 1 mM, 22% inhibition | Geobacillus stearothermophilus | |
| Fe2+ | 10 mM, 37% inhibition | Geobacillus stearothermophilus | |
| Hg2+ | 0.1 mM, 30% inhibition | Geobacillus stearothermophilus | |
| Mn2+ | 1 mM, 38% inhibition | Geobacillus stearothermophilus | |
| N-bromosuccinimide | xylan protects from inactivation | Geobacillus stearothermophilus | |
| Ni2+ | 10 mM, 49% inhibition | Geobacillus stearothermophilus | |
| Zn2+ | - |
Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Geobacillus stearothermophilus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Geobacillus stearothermophilus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41900 | - |
gel filtration | Geobacillus stearothermophilus |
| 43000 | - |
1 * 43000, SDS-PAGE | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
| Geobacillus stearothermophilus T-6 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-beta-D-xylan + H2O | - |
Geobacillus stearothermophilus | additional information | xylose + xylobiose | ? | |
| 1,4-beta-D-xylan + H2O | - |
Geobacillus stearothermophilus T-6 | additional information | xylose + xylobiose | ? | |
| carboxymethylcellulose + H2O | very low activity | Geobacillus stearothermophilus | ? | - |
? | |
| carboxymethylcellulose + H2O | very low activity | Geobacillus stearothermophilus T-6 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Geobacillus stearothermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 85 | 45°C: 28% of maximal activity, 85°C: 74% of maximal activity | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
pH 7.0, 10 h, stable, pH 9.0, half-life is 6 h | Geobacillus stearothermophilus |
| 70 | - |
pH 7.0, half-life is 14.5 h | Geobacillus stearothermophilus |
| 75 | - |
pH 7.0, half-life is 20 min | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Geobacillus stearothermophilus |
| 6.5 | 7 | - |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | pH 5.0: about 45% of maximal activity, pH 8.0: about 55% of maximal activity, pH 10.0: about 60% of maximal activity, pH 11.0: about 45% of maximal activity | Geobacillus stearothermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
65°C, stable for more than 10 h | Geobacillus stearothermophilus |
| 9 | - |
half-life: 6 h | Geobacillus stearothermophilus |
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