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Literature summary for 3.2.1.8 extracted from
- Some properties of an endo-1,4-beta-D-xylanase from the ligniperdous fungus Trametes hirsuta (1978), Folia Microbiol. (Praha), 23, 202-209.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Hydroxy-5-nitrobenzyl bromide | 10 mM, 94% inhibition | Trametes hirsuta |  |
| Ag+ | - |
Trametes hirsuta | |
| Hg2+ | - |
Trametes hirsuta | |
| iodoacetate | 10 mM, 13% inhibition | Trametes hirsuta | |
| N-Acetylimidazole | 100 mM, 79% inhibition | Trametes hirsuta | |
| N-bromosuccinimide | 5 mM, 82% inhibition | Trametes hirsuta | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Trametes hirsuta |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Trametes hirsuta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | 24000 | gel filtration | Trametes hirsuta |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes hirsuta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trametes hirsuta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-methylglucurono-D-xylan + H2O | - |
Trametes hirsuta | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, stable | Trametes hirsuta |
| 65 | - |
- |
Trametes hirsuta |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 75 | 30°C: about 50% of maximal activity, 75°C: about 30% of maximal activity | Trametes hirsuta |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
10 min, stable | Trametes hirsuta |
| 45 | - |
30 min, stable up to | Trametes hirsuta |
| 50 | - |
30 min, stable | Trametes hirsuta |
| 65 | - |
30 min, 90% loss of activity | Trametes hirsuta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Trametes hirsuta |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | 40°C, 10 min, stable | Trametes hirsuta |
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