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Literature summary for 3.2.1.8 extracted from

  • Lee, S.F.; Forsberg, C.W.; Rattray, J.B.
    Purification and characterization of two endoxylanases from Clostridium acetobutylicum ATCC 824 (1987), Appl. Environ. Microbiol., 53, 644-650.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 95% inhibition Clostridium acetobutylicum
EDTA unaffected by 5 mM EDTA Clostridium acetobutylicum
Hg2+ 1 mM, complete inhibition of xylanase A and B Clostridium acetobutylicum
Pb2+ 1 mM, 60% inhibition of xylanase A Clostridium acetobutylicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Clostridium acetobutylicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29500
-
xylanase B, gel filtration Clostridium acetobutylicum
29900
-
1 * 29900, xylanase B, SDS-PAGE Clostridium acetobutylicum
63000
-
xylanase A, gel filtration Clostridium acetobutylicum
65000
-
1 * 65000, xylanase A, SDS-PAGE Clostridium acetobutylicum

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum
-
ATCC 824
-

Purification (Commentary)

Purification (Comment) Organism
xylanase A and xylanase B Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4
-
xylanase A Clostridium acetobutylicum
6.59
-
xylanase B Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O larchwood xylan Clostridium acetobutylicum additional information xylanase A hydrolyzes larchwood xylan randomly, yielding xylohexaose, xylopentaose, xylotetraose, xylotriose and xylobiose. Xylanase B hydrolyzes xylan randomly with xylotriose and xylobiose as end products ?
lichenan + H2O
-
Clostridium acetobutylicum ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 65000, xylanase A, SDS-PAGE Clostridium acetobutylicum
monomer 1 * 29900, xylanase B, SDS-PAGE Clostridium acetobutylicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
xylanase A Clostridium acetobutylicum
5.5 6 xylanase B Clostridium acetobutylicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 7 xylanase B, stable Clostridium acetobutylicum
5.5 7 xylanase A, stable Clostridium acetobutylicum