Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM, 95% inhibition | Clostridium acetobutylicum | |
EDTA | unaffected by 5 mM EDTA | Clostridium acetobutylicum | |
Hg2+ | 1 mM, complete inhibition of xylanase A and B | Clostridium acetobutylicum | |
Pb2+ | 1 mM, 60% inhibition of xylanase A | Clostridium acetobutylicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Clostridium acetobutylicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29500 | - |
xylanase B, gel filtration | Clostridium acetobutylicum |
29900 | - |
1 * 29900, xylanase B, SDS-PAGE | Clostridium acetobutylicum |
63000 | - |
xylanase A, gel filtration | Clostridium acetobutylicum |
65000 | - |
1 * 65000, xylanase A, SDS-PAGE | Clostridium acetobutylicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium acetobutylicum | - |
ATCC 824 | - |
Purification (Comment) | Organism |
---|---|
xylanase A and xylanase B | Clostridium acetobutylicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.4 | - |
xylanase A | Clostridium acetobutylicum |
6.59 | - |
xylanase B | Clostridium acetobutylicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-beta-D-xylan + H2O | larchwood xylan | Clostridium acetobutylicum | additional information | xylanase A hydrolyzes larchwood xylan randomly, yielding xylohexaose, xylopentaose, xylotetraose, xylotriose and xylobiose. Xylanase B hydrolyzes xylan randomly with xylotriose and xylobiose as end products | ? | |
lichenan + H2O | - |
Clostridium acetobutylicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, xylanase A, SDS-PAGE | Clostridium acetobutylicum |
monomer | 1 * 29900, xylanase B, SDS-PAGE | Clostridium acetobutylicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
xylanase A | Clostridium acetobutylicum |
5.5 | 6 | xylanase B | Clostridium acetobutylicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7 | xylanase B, stable | Clostridium acetobutylicum |
5.5 | 7 | xylanase A, stable | Clostridium acetobutylicum |