Literature summary for 3.2.1.78 extracted from

Panwar, D.; Kaira, G.S.; Kapoor, M.
Cross-linked enzyme aggregates (CLEAs) and magnetic nanocomposite grafted CLEAs of GH26 endo-beta-1,4-mannanase Improved activity, stability and reusability (2017), Int. J. Biol. Macromol., 105, 1289-1299 .

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) cells	Bacillus sp. CFR1601

General Stability

General Stability	Organism
enzyme immobilization using cross-linked aggregated form (MB-C) and chitosan magnetic nanocomposites of MB-C leads to 73.2% higher activity (pH 4.0-9.0), up to 95.6% higher stability (pH 3.0-10.0, 9 h incubation at room temperature), up to 15°C higher optimal temperature, higher stability (up to 83%) in the presence of solvents and up to 1.62fold higher deactivation energy, as compared to the free enzyme. Immobilized enzymes are able to repeatedly hydrolyze locust bean gum till 12 cycles	Bacillus sp. CFR1601

Organism

enzyme immobilization using cross-linked aggregated form (MB-C) and chitosan magnetic nanocomposites of MB-C leads to 73.2% higher activity (pH 4.0-9.0), up to 95.6% higher stability (pH 3.0-10.0, 9 h incubation at room temperature), up to 15°C higher optimal temperature, higher stability (up to 83%) in the presence of solvents and up to 1.62fold higher deactivation energy, as compared to the free enzyme. Immobilized enzymes are able to repeatedly hydrolyze locust bean gum till 12 cycles

Bacillus sp. CFR1601

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. CFR1601	A0A0A1E3J1	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Bacillus sp. CFR1601

Purification (Commentary)

Purification (Comment)	Organism
phenyl Sepharose column chromatography	Bacillus sp. CFR1601

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
locust bean gum + H2O	-	Bacillus sp. CFR1601	mannobiose + mannotriose + mannotetraose	-	?

Synonyms

Synonyms	Comment	Organism
endo-beta-1,4-mannanase	-	Bacillus sp. CFR1601
ManB-1601	-	Bacillus sp. CFR1601

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	85	the free enzyme retains 42.5% residual activity after 35 min at 70-75°C. The half-lives of the free enzyme at 70, 75, 80 and 85°C are 138.6, 77, 24.7 and 25.6 min, respectively	Bacillus sp. CFR1601