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Literature summary for 3.2.1.78 extracted from

  • Ueda, M.; Hirano, Y.; Fukuhara, H.; Naka, Y.; Nakazawa, M.; Sakamoto, T.; Ogata, Y.; Tamada, T.
    Gene cloning, expression, and X-ray crystallographic analysis of a beta-mannanase from Eisenia fetida (2018), Enzyme Microb. Technol., 117, 15-22 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris nGS115 cells Eisenia fetida

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 1 M ammonium citrate, 15% (v/v) isopropanol, and 0.1M Tris-HCl (pH 8.5) Eisenia fetida

Organism

Organism UniProt Comment Textmining
Eisenia fetida A0A3B6UEQ6
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Eisenia fetida

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, HisTrap column chromatography, HiTrap DEAE column chromatography, and Superdex 75 gel filtration Eisenia fetida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-mannan + H2O
-
Eisenia fetida mannobiose + mannotriose
-
?
guar gum + H2O 12.2% activity compared to locust bean gum Eisenia fetida ?
-
?
konjac powder + H2O 33.5% activity compared to locust bean gum Eisenia fetida ?
-
?
locust bean gum + H2O 100% activity Eisenia fetida ?
-
?
mannohexaose + H2O
-
Eisenia fetida mannotriose + mannotetraose + mannopentaose + mannobiose
-
?
mannopentaose + H2O
-
Eisenia fetida mannotriose + mannobiose + mannotetraose
-
?
mannotetraose + H2O
-
Eisenia fetida mannotriose + mannobiose
-
?
additional information no activity with mannotriose, mannobiose, starch, carboxy methylcellulose, and birchwood xylan Eisenia fetida ?
-
?

Subunits

Subunits Comment Organism
? x * 39000, SDS-PAGE Eisenia fetida

Synonyms

Synonyms Comment Organism
Beta-mannanase
-
Eisenia fetida
endo-1,4-beta-mannanase
-
Eisenia fetida
Man
-
Eisenia fetida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Eisenia fetida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 about 40% activity at 40°C, about 65% activity at 50°C, 100% activity at 60°C, about 90% activity at 70°C, about 25% activity at 80°C Eisenia fetida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 50 the enzyme retains 100% activity after 30 min at 10-50°C, at 60°C the activity drops to about 50% Eisenia fetida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Eisenia fetida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 the enzyme activity is most stable at pH 6.0-10.0 Eisenia fetida