Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris nGS115 cells | Eisenia fetida |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 1 M ammonium citrate, 15% (v/v) isopropanol, and 0.1M Tris-HCl (pH 8.5) | Eisenia fetida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eisenia fetida | A0A3B6UEQ6 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Eisenia fetida |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, HisTrap column chromatography, HiTrap DEAE column chromatography, and Superdex 75 gel filtration | Eisenia fetida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,4-mannan + H2O | - |
Eisenia fetida | mannobiose + mannotriose | - |
? | |
guar gum + H2O | 12.2% activity compared to locust bean gum | Eisenia fetida | ? | - |
? | |
konjac powder + H2O | 33.5% activity compared to locust bean gum | Eisenia fetida | ? | - |
? | |
locust bean gum + H2O | 100% activity | Eisenia fetida | ? | - |
? | |
mannohexaose + H2O | - |
Eisenia fetida | mannotriose + mannotetraose + mannopentaose + mannobiose | - |
? | |
mannopentaose + H2O | - |
Eisenia fetida | mannotriose + mannobiose + mannotetraose | - |
? | |
mannotetraose + H2O | - |
Eisenia fetida | mannotriose + mannobiose | - |
? | |
additional information | no activity with mannotriose, mannobiose, starch, carboxy methylcellulose, and birchwood xylan | Eisenia fetida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE | Eisenia fetida |
Synonyms | Comment | Organism |
---|---|---|
Beta-mannanase | - |
Eisenia fetida |
endo-1,4-beta-mannanase | - |
Eisenia fetida |
Man | - |
Eisenia fetida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Eisenia fetida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | about 40% activity at 40°C, about 65% activity at 50°C, 100% activity at 60°C, about 90% activity at 70°C, about 25% activity at 80°C | Eisenia fetida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | the enzyme retains 100% activity after 30 min at 10-50°C, at 60°C the activity drops to about 50% | Eisenia fetida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Eisenia fetida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | the enzyme activity is most stable at pH 6.0-10.0 | Eisenia fetida |