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Literature summary for 3.2.1.78 extracted from
- A thermophilic beta-mannanase from Neosartorya fischeri P1 with broad pH stability and significant hydrolysis ability of various mannan polymers (2015), Food Chem., 173, 283-289.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Aspergillus fischeri |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene man5P1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of extracellular enzyme in Pichia pastoris strain GS115 | Aspergillus fischeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Aspergillus fischeri | |
| Co2+ | - |
Aspergillus fischeri | |
| Cu2+ | - |
Aspergillus fischeri | |
| EDTA | - |
Aspergillus fischeri | |
| Fe3+ | - |
Aspergillus fischeri | |
| K+ | - |
Aspergillus fischeri | |
| Li+ | - |
Aspergillus fischeri | |
| Mg2+ | - |
Aspergillus fischeri | |
| additional information | the purified recombinant enzyme has a strong resistance to SDS and Ag+ and proteases pepsin and trypsin | Aspergillus fischeri | |
| Ni2+ | - |
Aspergillus fischeri | |
| SDS | - |
Aspergillus fischeri | |
| Zn2+ | - |
Aspergillus fischeri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | recombinant enzyme, Km for locust bean gum is 0.83 ± 0.2 mg/ml, pH 4.0, 80°C | Aspergillus fischeri |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme contains a putative 19-residue signal peptide at the N-terminus | Aspergillus fischeri | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | poor effects by Na+, Ca2+, and Mn2+ | Aspergillus fischeri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39500 | - |
x * 39500, about, sequence calculation, x * 43000, recombinant glycosylated enzyme, SDS-PAGE, x * 40000, recombinant deglycosylated enzyme, SDS-PAGE | Aspergillus fischeri |
| 40000 | - |
x * 39500, about, sequence calculation, x * 43000, recombinant glycosylated enzyme, SDS-PAGE, x * 40000, recombinant deglycosylated enzyme, SDS-PAGE | Aspergillus fischeri |
| 43000 | - |
x * 39500, about, sequence calculation, x * 43000, recombinant glycosylated enzyme, SDS-PAGE, x * 40000, recombinant deglycosylated enzyme, SDS-PAGE | Aspergillus fischeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aspergillus fischeri | the enzyme is highly active towards galactomannan and glucomannan, and exhibits classic endo-activity producing a mixture of mannooligosaccharides | ? | - |
? | |
| additional information | Aspergillus fischeri P1 | the enzyme is highly active towards galactomannan and glucomannan, and exhibits classic endo-activity producing a mixture of mannooligosaccharides | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fischeri | - |
gene man5P1 | - |
| Aspergillus fischeri P1 | - |
gene man5P1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme contains two putative N-glycosylation sites and no O-glycosylation site, deglycosylation by endo-beta-N-acetylglucosaminidase H | Aspergillus fischeri |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant extracellular enzyme from Pichia pastoris strain GS115 culture supernatant by ultrafiltration and anion exchange chromatography | Aspergillus fischeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1703 | - |
purified recombinant enzyme, substrate locust bean gum, pH 4.0, 80°C | Aspergillus fischeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| galactomannan + H2O | different contents of glucose and mannose, overview | Aspergillus fischeri | mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose | product ratio overview | ? | |
| guar gum + H2O | - |
Aspergillus fischeri | mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose | product ratio overview | ? | |
| locust bean gum + H2O | - |
Aspergillus fischeri | mannose + mannobiose + mannotriose + mannotetraose + galactose-linked mannotetraose + mannopentaose + mannohexaose | product ratio overview | ? | |
| mannohexaose + H2O | - |
Aspergillus fischeri | ? | - |
? | |
| mannopentaose + H2O | - |
Aspergillus fischeri | ? | - |
? | |
| mannopentaose + H2O | - |
Aspergillus fischeri P1 | ? | - |
? | |
| mannotetraose + H2O | - |
Aspergillus fischeri | ? | - |
? | |
| mannotetraose + H2O | - |
Aspergillus fischeri P1 | ? | - |
? | |
| mannotriose + H2O | - |
Aspergillus fischeri | ? | - |
? | |
| mannotriose + H2O | - |
Aspergillus fischeri P1 | ? | - |
? | |
| additional information | the enzyme is highly active towards galactomannan and glucomannan, and exhibits classic endo-activity producing a mixture of mannooligosaccharides | Aspergillus fischeri | ? | - |
? | |
| additional information | the enzyme has no exo-activity and is a typical endo-acting beta-mannanase, substrate specificity, overview. No activity with mannobiose, beechwood xylan, barley beta-glucan, 4-nitrophenyl beta-D-mannoside, and carboxymethyl cellulose | Aspergillus fischeri | ? | - |
? | |
| additional information | the enzyme is highly active towards galactomannan and glucomannan, and exhibits classic endo-activity producing a mixture of mannooligosaccharides | Aspergillus fischeri P1 | ? | - |
? | |
| additional information | the enzyme has no exo-activity and is a typical endo-acting beta-mannanase, substrate specificity, overview. No activity with mannobiose, beechwood xylan, barley beta-glucan, 4-nitrophenyl beta-D-mannoside, and carboxymethyl cellulose | Aspergillus fischeri P1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39500, about, sequence calculation, x * 43000, recombinant glycosylated enzyme, SDS-PAGE, x * 40000, recombinant deglycosylated enzyme, SDS-PAGE | Aspergillus fischeri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Beta-mannanase | - |
Aspergillus fischeri |
| Man5P1 | - |
Aspergillus fischeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
recombinant enzyme | Aspergillus fischeri |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | recombinant enzyme, over 20% of maximal activity within this range | Aspergillus fischeri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the glycosylation status of the enzyme has no effect on the thermostability | Aspergillus fischeri |
| 60 | - |
purified recombinant enzyme, 6 h, over 80% activity remaining | Aspergillus fischeri |
| 70 | - |
purified recombinant enzyme, 80% activity remaining after 1 h, inactivation after 2 h | Aspergillus fischeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
recombinant enzyme | Aspergillus fischeri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 9 | recombinant enzyme, over 20% of maximal activity at pH 2.0 and pH 9.0 | Aspergillus fischeri |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 12 | purified recombinant enzyme, 1 h, over 65% activity remaining | Aspergillus fischeri |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus fischeri | sequence calculation | - |
5.93 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family 5, GH5 | Aspergillus fischeri |
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Release 2023.1 (January 2023)
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