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Literature summary for 3.2.1.78 extracted from
- Cloning and biochemical characterization of an endo-1,4-beta-mannanase from the coffee berry borer Hypothenemus hampei (2013), BMC Res. Notes, 6, 333.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | the enzyme is a target for the development of inhibitor molecules to be used in pest control strategies to reduce the serious damage caused by the coffee berry borer during coffee cultivation | Hypothenemus hampei |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene HhMan, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of C-terminally His6-tagged extracellular enzyme in and secretion from Pichia pastoris strain GS115 | Hypothenemus hampei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-nitrophenyl-thio-beta-D-mannopyranoside | - |
Hypothenemus hampei |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km is 2.074 mg/ml with guar gum for the recombinant enzyme at pH 5.5 and 30°C | Hypothenemus hampei |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Hypothenemus hampei | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 35500, recombinant His6-tagged enzyme, SDS-PAGE, x * 33000, about, enzyme without signal sequence, sequence calculation | Hypothenemus hampei |
| 35500 | - |
x * 35500, recombinant His6-tagged enzyme, SDS-PAGE, x * 33000, about, enzyme without signal sequence, sequence calculation | Hypothenemus hampei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Hypothenemus hampei | the enzyme hydrolyzes high-molecular weight polysaccharides with beta-1,4-glycosidic bonds | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hypothenemus hampei | - |
gene HhMan | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guar gum + H2O | - |
Hypothenemus hampei | ? | - |
? | |
| locust bean gum + H2O | - |
Hypothenemus hampei | ? | - |
? | |
| additional information | the enzyme hydrolyzes high-molecular weight polysaccharides with beta-1,4-glycosidic bonds | Hypothenemus hampei | ? | - |
? | |
| additional information | main by products of the hydrolysis of both guar and locust bean gum are mannose and manno-oligosaccharides of different sizes (mannobiose, mannotriose and mannotetraose). Enzyme mode of action involving a transglycosylation reaction in the mechanism that allows the enzyme to hydrolyze this manno-oligosaccharide. No activity with mannobiose and mannotriose | Hypothenemus hampei | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35500, recombinant His6-tagged enzyme, SDS-PAGE, x * 33000, about, enzyme without signal sequence, sequence calculation | Hypothenemus hampei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,4-beta-mannanase | - |
Hypothenemus hampei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
recombinant enzyme | Hypothenemus hampei |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 50 | approximately 70% between 10°C and 20°C, 60% activity at 40°C, 30% activity at 50°C | Hypothenemus hampei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50.87 | - |
guar gum | pH 5.5, 30°C, recombinant enzyme | Hypothenemus hampei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
recombinant enzyme | Hypothenemus hampei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | 95% activity at pH 5.0 and 75% activity at pH 4.0, pH 6.0 and pH 7.0, 50% at pH 7.5 and pH 8.0 | Hypothenemus hampei |
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