Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | an assembly of ManB with mini-CbpA, which contains a carbohydrate-binding module that provides proximity to insoluble substrates, increases the activity toward galactomannan from locust bean gum and guar gum 1.7- and 2.0fold over those without mini-CbpA | Clostridium cellulovorans |
Application | Comment | Organism |
---|---|---|
degradation | when assembled with the mini-CbpA, which contains a carbohydrate-binding module that provides proximity to insoluble substrates, a mixture of endoglucanase E and ManB at a molar ratio of 1:2 shows the highest synergistic effect of 2.4fold on locust bean gum degradation. The mixture at a ratio of 1:3 shows the highest synergistic effect of 2.8fold on guar gum | Clostridium cellulovorans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Clostridium cellulovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE, recombinant protein including His-tag | Clostridium cellulovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium cellulovorans | - |
member of glycoyl hydrolase family 26 | - |
Purification (Comment) | Organism |
---|---|
- |
Clostridium cellulovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.2 | - |
substrate guar gum, pH 7.0, 40°C | Clostridium cellulovorans |
12.2 | - |
substrate locust bean gum, pH 7.0, 40°C | Clostridium cellulovorans |
18.5 | - |
substrate glucomannan, pH 7.0, 40°C | Clostridium cellulovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactoglucomannan + H2O | - |
Clostridium cellulovorans | ? | - |
? | |
guar gum + H2O | - |
Clostridium cellulovorans | ? | - |
? | |
locust bean gum + H2O | - |
Clostridium cellulovorans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE, recombinant protein including His-tag | Clostridium cellulovorans |
Synonyms | Comment | Organism |
---|---|---|
ManB | - |
Clostridium cellulovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Clostridium cellulovorans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 30 | more than 80% of maximum activity | Clostridium cellulovorans |
50 | - |
rapid decrease in activity above | Clostridium cellulovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Clostridium cellulovorans |