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Literature summary for 3.2.1.78 extracted from
- Expression of Bacillus subtilis MA139 beta-mannanase in Pichia pastoris and the enzyme characterization (2010), Appl. Biochem. Biotechnol., 160, 1362-1370.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | optimization of mannanase gene for expression in Pichia pastoris by substitution of 258 nucleotides with their corresponding counterparts according to the codon usage in Pichia pastoris, which has no change on the beta-mannanase amino acid sequence. Compared to the activity of wild-type, the expression enzyme of the optimized beta-mannanase gene acquires approximately 35% more activity | Bacillus subtilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Bacillus subtilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | optimization of mannanase gene for expression in Pichia pastoris by substitution of 258 nucleotides with their corresponding counterparts according to the codon usage in Pichia pastoris, which has no change on the beta-mannanase amino acid sequence | Bacillus subtilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 38% residual activity | Bacillus subtilis |  |
| EDTA | 64% residual activity | Bacillus subtilis | |
| Mn2+ | 53% residual activity | Bacillus subtilis | |
| Zn2+ | 79% residual activity | Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | B5LX62 | beta-mannanase | - |
| Bacillus subtilis MA139 | B5LX62 | beta-mannanase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| galactomannan + H2O | - |
Bacillus subtilis | ? | - |
? | |
| galactomannan + H2O | - |
Bacillus subtilis MA139 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Beta-mannanase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
about 50% of maximum activity | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
30 min, 60% rsidual activity | Bacillus subtilis |
| 60 | - |
30 min, complete loss of activity | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | more than 60% of maximum activity | Bacillus subtilis |
| 8 | - |
50% of maximum activity | Bacillus subtilis |
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