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Literature summary for 3.2.1.78 extracted from

  • van Zyl, P.J.; Moodley, V.; Rose, S.H.; Roth, R.L.; van Zyl, W.H.
    Production of the Aspergillus aculeatus endo-1,4-beta-mannanase in A. niger (2009), J. Ind. Microbiol. Biotechnol., 36, 611-617.
    View publication on PubMed
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Application

Application Comment Organism
synthesis production of enzyme by expression in Aspergillus niger under control of the Aspergillus niger glyceraldehyde-3-phosphate dehydrogenase promoter gpdP and the Aspergillus awamori glucoamylase terminator glaAT. The glucose concentration and the organic nitrogen source have an effect on both the volumetric enzyme activity and the specific enzyme activity. The highest mannanase activity levels of 16596 nkat ml-1 and 574 nkat mg-1 dcw are obtained for Aspergillus niger when cultivated in a process-viable medium containing corn steep liquor as the organic nitrogen source and high glucose concentrations Aspergillus aculeatus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus niger Aspergillus aculeatus

Organism

Organism UniProt Comment Textmining
Aspergillus aculeatus
-
-
-
Aspergillus aculeatus MRC11624
-
-
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75 80
-
 Aspergillus aculeatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 5 h, more than 50% residual activity Aspergillus aculeatus
80
-
 2 min, complete loss of activity Aspergillus aculeatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8
-
-
 Aspergillus aculeatus